Description:
GenScript's 5X Taq Blue Master Mix is a ready-to-use mixture of high-quality Taq DNA polymerase, salts, magnesium, loading dye, and dNTPs, designed for efficient amplification of templates in reactions. The user need only add a template and primers, cutting reaction setup time by half. It allows direct loading of the PCR reaction product onto agarose gels. The 5X Taq Blue Master Mix comes as one vial containing about 1 ml of solution, enough for 100 reactions (10 μl each in a 50 μl system). 5X Taq Blue Master Mix may be stored at either 4°C or -20°C. No detectable reduction of PCR performance is observed after storage at 25°C for four weeks.
Contents:
50 mM Tris-HCl (pH 9.0), 250 mM KCl, 7.5 mM MgCl2, 1000 μM dNTP Mix, 0.5% Triton X-100, 0.0125% Bromophenol Blue, 250 U recombinant Taq DNA Polymerase/ml and stabilizer.
Storage:
Store at -20°C.
EXAMPLE:
PROTOCOL: 20070529053342 (PDF)
MSDS: 20080228033919 (PDF)
