Description:
GenScript RealStart Taq DNA Polymerase is designed for highly specific PCR, such as those performed with the HotStart PCR technique, which enhances the specificity, sensitivity, and yield of PCR DNA amplification (1, 2). In general, HotStart PCR methods reduce or eliminate non-specific and unwanted DNA amplification, such as that of primer-dimers, and other artifacts during PCR reaction set-up and before real PCR starts. GenScript RealStart Taq DNA polymerase has been engineered and formulated to function only in true PCR cycles, eliminating non-specific amplification.
GenScript RealStart Taq DNA Polymerase is formulated in such a way that no prolonged heating or denaturing step is necessary, making RealStart Taq DNA Polymerase more convenient and easy to use.
Applications:
The applications of GenScript RealStart Taq DNA Polymerase include the following:
- High-specificity PCR
- Amplification of complex genomic or cDNA templates
- Amplification of DNA targets at low concentrations
- Multiplex PCR
- 3’ A-tailing of blunt-end DNA
Buffers:
RealStart Taq DNA Polymerase storage buffer is formulated as follows: 20 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 1 mM DTT, 0.01% Triton X-100, 50% glycerol.
Two 2X Reaction Buffers are included: 2X Reaction buffer I (with Mg2+) and 2X Reaction Buffer II (with Mg2+). Always use 2X Reaction Buffer I (with Mg2+) first. In some rare cases, 2X Reaction Buffer I may not work, then use 2X Reaction Buffer II.
Concentration and Unit Definition :
The product is supplied at a concentration of 5 units/μl. One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble fraction in 30 minutes at 74°C.
Shipping and Storage:
Store the product at -20 °C. The enzyme remains stable for at least 12 months if stored at -20°C. This product is shipped on blue ice.
PROTOCOL: 20080304215724 (PDF)
