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Quickclean 5M Miniprep Kit
Description
The Quickclean 5M Miniprep Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and other supplies for quick, reliable, high-quality plasmid DNA or cosmid DNA preparation from bacteria such as E. coli cell culture. Plasmid and cosmid DNA molecules are selectively adsorbed on to the QuickClean column, and other impurities such as proteins, salts and nucleotides are washed. Eluted in a small volume low-salt buffer with complete removal of contaminants and inhibitors, the purified plasmid or cosmid DNA is immediately ready for many downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation, and transfection.
Key Features:
- Easy to perform: QuickClean's simple and rapid procedure purifies DNA in five minutes.
- High capacity: Each column has a capacity of 20 µg plasmid DNA.
- High purity: The kit completely removes contaminants and inhibitors.
- Reproducible yields: Recovery is typically between 90% and 95% pure plasmid DNA, reproducible every time.
Figure 1. Comparison of preparation time with QuickClean 5M MiniPrep Kit vs. other brand kit.
Applications
The QuickClean 5M Miniprep Kit enables the preparation of high-quality plasmid DNA ready for most downstream applications such as:
- PCR and cloning
- Restriction enzyme digestion
- Transformation
- Sequencing
- In vitro translation
Order:
Technical Manual:
Components:
MSDS: 20050711103728 (PDF) PROTOCOL: 20080324014726 (PDF) Examples:A. Plasmid DNA preparation from E. coli bearing high copy number plasmid. The QuickClean 5M Miniprep Kit is here compared to a commercially available kit (Competitor A) for rapid plasmid purification from E. coli bearing high copy number plasmid. Both kits are used in compliance with the protocols provided by the manufacturers. The results are shown in Figure 1.  Figure 1.A kit from competitor A and QuickClean 5M Miniprep Kit were used for rapid plasmid purification from E. coli bearing high copy number plasmid following the protocols provided by the manufacturers. Six minipreps were performed using each kit. The same volume of cell cuture was used in each miniprep. Figure 1 shows that both kits enabled the rapid purification of plasmid. The Genscript Quickclean 5M Miniprep Kit, however, purified the plasmid more reliably and with a higher yield from each miniprep. B.Plasmid DNA preparation from E. coli bearing low copy number plasmid. The QuickClean 5M Miniprep Kit is also compared with the kit from Competitor A for rapid plasmid purification from E. coli bearing low copy number plasmid. Both kits have been used in compliance with the protocols provided by the manufacturers. The results were shown in Figure 2.  Figure 2. The kit from competitor A and QuickClean 5M Miniprep Kit were used for rapid plasmid purification from E. coli bearing low copy number plasmid following the protocols provided by the manufacturers. Six minipreps were performed using each kit. The same volume of cell cuture was used in each miniprep. Figure 2 shows that both kits enabled the rapid purification of plasmid from E. coli bearing low copy number plasmid. With GenScript Quickclean 5M Miniprep Kit, however, the plasmid was purified reproducibly with higher yield from each miniprep. |