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Note:
This product has been discontinued!
The improved replacement is  L00419

QuickClean 5M PCR Purification Kit


Description
The QuickClean 5M PCR Purification Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and collection tubes for the fast and reliable extraction of DNA from PCR reactions and for DNA cleanup in enzymatic reactions. In only five minutes, QuickClean can recover up to 20 µg of single- or double-stranded DNA product. DNA molecules ranging in size from 70 to 10,000 base pairs are adsorbed onto the QuickClean Column while impurities such as enzymes and proteins, small DNA fragments, dyes, salts, nucleotides and short oligonucleotides (such as primers and probes) are washed away.

Eluted in a small volume of low-salt buffer with complete removal of contaminants and inhibitors, the purified DNA is immediately ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation and transfection.

Key Features

Applications
The QuickClean 5M PCR Purification Kit enables the fast purification of high-quality DNA products from PCR reactions and enzymatic reactions. The extracted DNA is immediately ready for further downstream applications such as the following:
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Technical Manual


Components:

Components L00198
Binding solution I 2 x 75 ml 
Wash solution 55 ml 
Elution buffer 25 ml 
Genprep columns 250 
2 ml Collection tubes 250 
Protocol 

MSDS:  20050711103550  (PDF)
PROTOCOL:  20080324234337  (PDF)


Examples
The QuickClean 5M PCR Purification Kit is here compared to a commercially available kit (Competitor A) for rapid PCR DNA product purification from PCR reactions. Both kits were used following the protocols provided by the manufacturers. The results are shown in Figure 1.

Figure 1. The QuickClean 5M Purification Kit and a kit from competitor A were used for PCR DNA purification from PCR reactions. Both kits were used in accordance with the protocols provided by the manufacturers. Two PCR DNA purifications were performed using each kit. The same volume of PCR material was used in each purification. Lane 1 shows unpurified material.

Related Products:


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M102O  DNA Marker: 100 bp DNA Ladder  500 Lanes $ 170.00
M102R  DNA Marker: Ready-to-UseTM 100 bp DNA Ladder  100 Lanes $ 55.00
M102R  DNA Marker: Ready-to-UseTM 100 bp DNA Ladder  500 Lanes $ 170.00
D0056  dNTP mixture, 10 mM each  0.5 ml $ 25.00



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