QuickClean 5M PCR Purification Kit

Description

The QuickClean 5M PCR Purification Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and collection tubes for the fast and reliable extraction of DNA from PCR reactions and for DNA cleanup in enzymatic reactions. In only five minutes, QuickClean can recover up to 20 �g of single- or double-stranded DNA product. DNA molecules ranging in size from 70 to 10,000 base pairs are adsorbed onto the QuickClean Column while impurities such as enzymes and proteins, small DNA fragments, dyes, salts, nucleotides and short oligonucleotides (such as primers and probes) are washed away.

Eluted in a small volume of low-salt buffer with complete removal of contaminants and inhibitors, the purified DNA is immediately ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation and transfection.

Key Features

Easy to perform: QuickClean's simple and rapid procedure purifies DNA in five minutes.
High capacity: Each column has a capacity of 20 �g DNA.
High purity: The kit completely removes contaminants and inhibitors.
Recovery is typically between 50% and 90%.

Applications

The QuickClean 5M PCR Purification Kit enables the fast purification of high-quality DNA products from PCR reactions and enzymatic reactions. The extracted DNA is immediately ready for further downstream applications such as the following:

PCR and cloning
Restriction enzyme digestion
Transformation
Sequencing
In vitro translation

Order:

Technical Manual

Download PDF file TM0182

Components:

Components	L00198
Binding solution I	2 x 75 ml
Wash solution	55 ml
Elution buffer	25 ml
Genprep columns	250
2 ml Collection tubes	250
Protocol	1

MSDS: 20050711103550 (PDF)
PROTOCOL: 20080324234337 (PDF)

Examples

The QuickClean 5M PCR Purification Kit is here compared to a commercially available kit (Competitor A) for rapid PCR DNA product purification from PCR reactions. Both kits were used following the protocols provided by the manufacturers. The results are shown in Figure 1.

Figure 1. The QuickClean 5M Purification Kit and a kit from competitor A were used for PCR DNA purification from PCR reactions. Both kits were used in accordance with the protocols provided by the manufacturers. Two PCR DNA purifications were performed using each kit. The same volume of PCR material was used in each purification. Lane 1 shows unpurified material.

Code	Name	Size	Price
E00007	Enzyme : Taq DNA Polymerase	1000 U	$ 60.00
E00007	Enzyme : Taq DNA Polymerase	50000 U	$ 2700.00
M102O	DNA Marker: 100 bp DNA Ladder	100 Lanes	$ 55.00
M102O	DNA Marker: 100 bp DNA Ladder	500 Lanes	$ 170.00
M102R	DNA Marker: Ready-to-Use^TM 100 bp DNA Ladder	100 Lanes	$ 55.00
M102R	DNA Marker: Ready-to-Use^TM 100 bp DNA Ladder	500 Lanes	$ 170.00
D0056	dNTP mixture, 10 mM each	0.5 ml	$ 25.00


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