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QuickClean 5M PCR Purification Kit

Description:

The QuickClean 5M PCR Purification Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and collection tubes for the fast and reliable extraction of DNA from PCR reactions and for DNA cleanup in enzymatic reactions. In only five minutes, QuickClean can recover up to 20 µg of single- or double-stranded DNA product. DNA molecules ranging in size from 70 to 10,000 base pairs are adsorbed onto the QuickClean Column while impurities such as enzymes and proteins, small DNA fragments, dyes, salts, nucleotides and short oligonucleotides (such as primers and probes) are washed away.

Eluted in a small volume of low-salt buffer with complete removal of contaminants and inhibitors, the purified DNA is immediately ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation and transfection.

Key Features:

• Easy to perform: QuickClean's simple and rapid procedure purifies DNA in five minutes.
• High capacity: Each column has a capacity of 20 µg DNA.
• High purity: The kit completely removes contaminants and inhibitors.
• Reproducible yields: Recovery is typically between 90% and 98%, reproducible every time.

Applications:

The QuickClean 5M PCR Purification Kit enables the fast purification of high-quality DNA products from PCR reactions and enzymatic reactions. The extracted DNA is immediately ready for further downstream applications such as the following:

• PCR and cloning
• Restriction enzyme digestion
• Transformation
• Sequencing
• In vitro translation

Order:

Cat.No. Name Size Price Quantity   L00198 QuickClean 5M PCR Purification Kit  250 preps $ 145.00

Technical Manual: Download PDF file TM0182 Components: Components L00198 Binding solution I  2 x 75 ml  Wash solution  55 ml  Elution buffer  25 ml  Genprep columns  250  2 ml Collection tubes  250  Protocol  1  MSDS:  20050711103550  (PDF) PROTOCOL:  20080324234337  (PDF) Examples: The QuickClean 5M PCR Purification Kit is here compared to a commercially available kit (Competitor A) for rapid PCR DNA product purification from PCR reactions. Both kits were used following the protocols provided by the manufacturers. The results are shown in Figure 1. Figure 1. The QuickClean 5M Purification Kit and a kit from competitor A were used for PCR DNA purification from PCR reactions. Both kits were used in accordance with the protocols provided by the manufacturers. Two PCR DNA purifications were performed using each kit. The same volume of PCR material was used in each purification. Lane 1 shows unpurified material.

Related Products:   Code Name Size Price Quantity L00193  QuickClean 5M Miniprep Kit  250 preps  $ 145.00 E00007  Enzyme : Taq DNA Polymerase  1000 U  $ 60.00 E00007  Enzyme : Taq DNA Polymerase  50000 U  $ 2700.00 M101O  DNA Marker: DNA KB Ladder - original format  100 Loading  $ 25.00 M101O  DNA Marker: DNA KB Ladder - original format  500 Loading  $ 85.00 M101R  DNA Marker: DNA KB Ladder - ready-to-useTM  100 Loading  $ 30.00 M101R  DNA Marker: DNA KB Ladder - ready-to-useTM  500 Loading  $ 95.00 M102O  DNA Marker: 100 bp DNA Ladder - original format  100 Loading  $ 44.00 M102O  DNA Marker: 100 bp DNA Ladder - original format  500 Loading  $ 170.00 M102R  DNA Marker: 100 bp DNA Ladder - ready-to-useTM  100 Loading  $ 50.00 M102R  DNA Marker: 100 bp DNA Ladder - ready-to-useTM  500 Loading  $ 180.00 D0056  dNTP mixture, 10 mM each  0.5 ml  $ 25.00

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