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Note:

This product has been discontinued! The improved replacement is  L00418

QuickClean 5M Gel Extraction Kit

Description

GenScript QuickClean 5M Gel Extraction Kit offers fast DNA extraction and purification from both standard and low-melt agarose gels. Up to 20 μg of double- or single-stranded DNA can be recovered from a solubilized gel slice in just five minutes. DNA molecules of anywhere between 70 and 10,000 base pairs absorb to the Genprep Column and contaminants and inhibitors such as agarose, dyes, and salts are washed away. Eluted in a small volume of low-salt buffer, the purified DNA is immediately ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation, and transfection.

Key Features

• Easy to perform: Simple and rapid procedure to purify DNA in 5 min from solubilized gel slice.
• Minimal preparation time: QuickClean's simple and rapid procedure purifies DNA in 5 - 10 minutes.
• High capacity: Each column has a capacity of 20 µg plasmid DNA.
• High purity: The kit completely removes contaminants and inhibitors.
• Reproducible yields: Recovery is typically between 90% and 95% pure plasmid DNA, reproducible every time.

Applications

The QuickClean 5M Gel Extraction Kit enables fast, high-quality extraction and purification of DNA from agarose gels. This DNA is immediately ready for further downstream applications such as the following:

• PCR and cloning
• Restriction enzyme digestion
• Transformation
• Sequencing
• in vitro translation

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Cat.No. Name Size Price Quantity

Technical Manual Download PDF file TM0183 Components: Components Binding Solution II  Wash Solution  Elution Buffer  Genprep Columns  2 ml Collection Tubes  Protocol  MSDS:  20050711103859  (PDF) PROTOCOL:  20050727135926  (PDF) Examples Shown below is a comparison of the QuickClean 5M Gel Extraction Kit to an anonymous competitor. Figure 1.The QuickClean 5M Gel Extraction Kit and a kit from Competitor A were to extract and purify DNA from agarose gels. Both kits were used in accordance with the protocols provided by the manufacturers. Two DNA purifications were performed using each kit. The same amount of DNA was loaded into four wells of the agarose gel used in this test. Lane 1 shows unpurified material.

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