You are here:  Products » PCR and Cloning » Direct PCR »  BacReadyTM Multiplex PCR System

BacReady^TM Multiplex PCR System

Description

BacReady^TM Multiplex PCR System is a powerful reagent kit for both rapid preparation and multiplex PCR amplification of bacteria genomic DNA and plasmid DNA. Genomic and plasmid DNA are directly released from bacteria (both gram-positive and gram-negative) cells in a single step by adding a proprietary reagent directly to bacteria samples without DNA purification. The genomic and plasmid DNA can then be used immediately in PCR amplification of multiple gene targets (up to >1,000) or stored at 4°C for future use (stable at least 6 months at 4°C).

Key features of genscript PCR systems:

• Easy to perform: very simple and rapid procedure to extract genomic DNA and plasmid DNA in a single step.
  
  
• High specificity: highly specific amplification of genomic DNA using "HotStart" Script^TM DNA polymerase (a genscript proprietary DNA polymerase).
  
  
• Multiplex PCR: up to >1,000 DNA sequences can be amplified using multiplex PCR primers.
  
  
• Super sensitivity: a single bacteria cell can be used to amplify both genomic DNA and plasmid DNA.
  

Applications

For genomic DNA prepared from: gram-positive or gram-negative bacteria.

For applications such as:

• SNP genotyping and mutation detection
• Target detection in transgenic plant
• DNA sequencing and cloning
• Quantitative PCR

Order:

Cat.No. Name Size Price Quantity   L00202 BacReadyTM Multiplex PCR System  100 Preps (without Enzyme) $ 111.00

Technical Manual: Download PDF file TM0191 Components: Components L00202 BR-A buffer  5 ml  BR-B buffer  0.2 ml  PCR-grade water  1 ml  MSDS:  20050711104519  (PDF) PROTOCOL:  20070410225700  (PDF) Application Examples: A. Gram-negative Bacteria Genomic DNA Preparation and PCR Amplification. To test genomic DNA preparation from gram-negative bacteria and PCR amplification, a pairs of primers specific for E. coli are selected to amplify part of 16S rRNA gene. The expected PCR product is 544 bp. And the sequences of the two primers used in the experiments are: Forward primer: 5’-GGAAGAAGCTTGCTTCTTTGCTGAC-3’ Reverse primer: 5’-AGCCCGGGGATTTCACATCTGACTTA-3’ Genomic DNAs were prepared from four different E. coli cultures and amplified using BacReadyTM Multiplex PCR system (with Enzyme) following the kit instructions. The results were shown in Figure 1. Figure 1. PCR analysis of genomic DNA prepared from bacteria samples. Genomic DNAs were prepared from 4 different E. coli cultures and amplified using BacReady(TM) Multiplex PCR kit following the kit instructions. M is 100 bp DNA marker lane. Lane 1 and 2 are culture No. 1; Lane 3 and 4 are culture No. 2, and so on. Lane 9 is a negative control. PCR products are 544 bp. B. Gram-positive Bacteria Genomic DNA Preparation and PCR Amplification. To test genomic DNA preparation from gram-positive bacteria and PCR amplification, a pairs of primers specific for gram-positive bacteria are selected to amplify part of rRNA gene. The expected PCR product is 355 bp. And the sequences of the two primers used in the experiments are: Forward primer: 5’-GCGRCTCTCTGGTCTGTA-3’ Reverse primer: 5’-GACGACAGCCATGCASCACCTGT-3’ Genomic DNAs were prepared from five different samples and amplified using BacReadyTM Multiplex PCR system (with Enzyme) following the kit instructions. The results were shown in Figure 2. Figure 2. Bacteria genomic DNA was prepared and amplified using BacReady(TM) Multiplex PCR System (with Enzyme). Five different samples were tested. Lane 1 and 2 were yogurt samples; Lane 3, 4 and 5 were bacteria samples picked from slants, they were Bacillus globigii, Bacillus ubtillus and Micrococcus leteus, respectively. Lane 6 is a negative control. PCR products are 355 bp. Figure 2 shows that all the five DNA samples prepared using the kit produced the expected PCR products. There is little difference between the PCR amplification from 5 samples. This demonstrates the high quality and reproducibility of BacReady(TM) PCR System.

Related Products:   Code Name Size Price Quantity L00194  TissueDirectTM Multiplex PCR System  100 Preps (without Enzyme)  $ 113.00 L00196  BloodReadyTM Multiplex PCR System  100 Preps (without Enzyme)  $ 111.00 L00197  BloodReadyTM Multiplex PCR System  100 Preps (with Enzyme)  $ 157.00 L00200  PlantDirectTM Multiplex PCR System  100 Preps (without Enzyme)  $ 139.00 L00201  PlantDirectTM Multiplex PCR System  100 Preps (with Enzyme)  $ 159.00 L00202  BacReadyTM Multiplex PCR System  100 Preps (without Enzyme)  $ 111.00 L00203  BacReadyTM Multiplex PCR System  100 Preps (with Enzyme)  $ 157.00 E00007  Enzyme : Taq DNA Polymerase  1000 U  $ 60.00 E00007  Enzyme : Taq DNA Polymerase  50000 U  $ 2700.00 D0056  dNTP mixture, 10 mM each  0.5 ml  $ 25.00

1	Custom Recombinant Protein Services : Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2	Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3	Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
4	Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5	Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.