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One-Step WesternTM Complete Kit Using TMB
Kit Description:
Based on GenScript's One-Step WesternTM blot technology, the One-Step WesternTM Complete Kit cuts total analysis time for Western and Dot blots down to just one hour. Both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB, are compatible with this kit.
The One-Step WesternTM Kit with TMB contains WestClearTM Nitrocellulose Membrane(0.2 µm, L00224A60) and ChomoSensorTM One-Solution TMB Substrate (L00222V60). This kit is also compatible with Chemiluminescent substrate.
Key Features :
Easy to perform: Fewer steps mean fewer chances for human error.
The signal can be observed without any darkroom or special development.
Low background: The ChromoSensorTM One-Solution TMB substrate is optimized to give very low background.
High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
Reproducible results: The kit produces highly reproducible results.
Needs far less optimization than the classical three-step method.
No secondary antibody is needed.
Applications:
The One-Step WesternTM Kit with TMB has applications that include the following:
Western-blot detection of housekeeping protein α-tubulin.
Western blot for the detection of α-tubulin using One-Step WesternTM Kit with TMB (L00205T) and Anti α-tubulin mAb (abcam, ab7291). 10 µg, 5.0 µg, 2.5 µg, 1.3 µg, 0.63 µg, and 0.32 µg of Hela Cell Lysate (BD Biosciences, 611449) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, and Lane 6, respectively. The blot was developed with the ChromoSensorTM One-Solution TMB substrate included in the kit.
