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One-Step WesternTM Basic Kit
Kit Description:
Based on GenScript's One-Step WesternTM Blot technology, the One-Step WesternTM Basic Kit cuts total analysis time for Western and Dot blots down to just one hour. Both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB, are compatible with this kit.
To obtain best results and reduce undesirable variations such as high background, we strongly recommend that the following reagents be used with One-Step WesternTM Basic Kit:
GenScript WestClearTM Nitrocellulose Membrane (L00224A60).
GenScript LumiSensorTM Chemiluminescent HRP Substrate Kit (L00221V60).
GenScript ChromoSensorTM One Solution TMB Substrate (L00222V60).
These kits are not recommended for use with antibodies against phosphoproteins.
Key Features:
- Easy to perform: Fewer steps mean fewer chances for human error.
- High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
- Reproducible results: The kit produces highly reproducible results.
- Needs far less optimization than the classical three-step method.
- No secondary antibody is needed.
Applications:
The One-Step WesternTM Basic Kit cuts hours off the following applications:
- Protein/antigen detection
- Evaluation of protein expression
- Antibody/antigen titration
- Analysis and comparison of primary antibody activity levels
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