One-Step Western^TM Complete Kit (Mouse)

Kit Description:

Key Features:

• Easy to perform: Fewer steps mean fewer chances for human error.
• High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
• Reproducible results: The kit produces highly reproducible results.
• Needs far less optimization than the classical three-step method.
• No secondary antibody is needed.

Applications:

• Protein/antigen detection
• Evaluation of protein expression
• Antibody/antigen titration
• Analysis and comparison of primary antibody activity levels

Order:

Cat.No. Name Size Price Quantity   L00205C One-Step WesternTM Complete Kit (Mouse)  1 Kit $ 275.00   L00205C One-Step WesternTM Complete Kit (Mouse)  5 Kit $ 962.50

Technical Manual: Download PDF file TM0202 Components: Components L00205C L00205C L00205C50 code      Application  For Mouse Primary Antibody    Pretreat A Solution  100 ml    Pretreat B Solution  100 ml    WB Solution  100 ml    10X Wash Solution  100 ml    WestClearTM Nitrocellulose Membrane  10 sheets    LumiSensorTM Chemiluminescent HRP Substrate Kit  2 X 30 ml    PROTOCOL:  20080226222850  (PDF) Examples: Comparison of One-Step WesternTM Blot detection with classical Western blot detection. Western blots for the detection of α-Tubulin protein from Hela cell lysate by both classical Western and One-Step WesternTM Complete Kit (Mouse) (L00205C10). 10 µg, 2.5 µg, 0.63 µg, and 0.16 µg of Hela cell lysate (GenScript, M0045) were loaded in different lanes as shown in the figure beloww. The classical Western blot was developed with ECL system (GE Healthcare, RPN2109). The classical Western blot was developed using the ECL system (GE Healthcare, RPN2109). The One-Step WesternTM Blot was developed using the LumiSensorTM Chemiluminescent HRP Substrate included in the kit. Dot blot detection of IL-8 protein. Dot blot for the detection of IL-8 protein using the One-Step WesternTM Complete Kit (Mouse) (L00205-C10) and Mouse Anti-Human IL-8 Monoclonal Antibody (GenScript, A00044). 120 ng, 40.0 ng, 13.3 ng, 7.4 ng, 4.4 ng, and 1.5 ng of IL-8 protein were spotted on the membrane. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit. Western blot detection of housekeeping protein GAPDH using monoclonal antibody. Western blot for the detection of GAPDH using the One-Step WesternTM Complete Kit (Mouse) (L00205-C10) and Mouse Anti-GAPDH Monoclonal Antibody (GenScript, A00084). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg, 0.16 µg, and 0.08 µg of Hela cell lysate (GenScript, M0045) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.