This product has been discontinued! The improved replacement is L00205C
One-Step WesternTM Complete Kit (Mouse)
Kit Description:
Based on GenScript's One-Step WesternTM blot technology, the One-step WesternTM Complete Kit reduces the total analysis time for a Western or Dot blot to just one hour. Both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB, are compatible with this kit.
The kit contains WestClearTM Nitrocellulose Membrane (0.2 µm, L00224A60) and LumiSensorTM Chemiluminescent HRP Substrate Kit (L00221V60), optimized for best results. WestClearTM Nitrocellulose Membrane and LumiSensorTM Chemiluminescent HRP Substrate Kit are also available separately.
Key Features:
Easy to perform: Fewer steps mean fewer chances for human error.
High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
Reproducible results: The kit produces highly reproducible results.
Needs far less optimization than the classical three-step method.
No secondary antibody is needed.
Applications:
The One-Step WesternTM Complete Kit cuts hours off the following applications:
Protein/antigen detection
Evaluation of protein expression
Antibody/antigen titration
Analysis and comparison of primary antibody activity levels
Comparison of One-Step WesternTM blot detection with classical Western blot detection.
Western blots for the detection of α-Tubulin protein from Hela cell lysate by both classical Western and One-Step WesternTM(L00205C10). 10 µg, 2.5 µg, 0.63 µg, and 0.16 µg of Hela cell lysate (GenScript, M00045) were loaded in different lanes as shown in the figure beloww. The classical Western blot was developed with ECL system (GE Healthcare, RPN2109). The classical Western blot was developed using the ECL system (GE Healthcare, RPN2109). The One-Step Western blot was developed using the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.
Dot blot detection of IL-8 protein.
Dot blot for the detection of IL-8 protein using the One-Step WesternTM Kit (L00205-C10) and Mouse Anti IL-8 mAb (GenScript, A00044). 120 ng, 40.0 ng, 13.3 ng, 7.4 ng, 4.4 ng, and 1.5 ng of IL-8 protein were spotted on the membrane. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.
Western blot detection of housekeeping protein GAPDH using monoclonal antibody.
Western blot for the detection of GAPDH using the One-Step WesternTM Kit (L00205-C10) and Mouse Anti GAPDH mAb (GenScript, A00084). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg, 0.16 µg, and 0.08 µg of Hela cell lysate (GenScript, M00045) were were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.
