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Glutathione Resin

Description:

GenScript Glutathione Resin (L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. Table 1 lists the main characteristics of Glutathione Resin.

The GST Fusion Protein Purification Kit (L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript Protein Expression and Purification Kit (L00208) provide all the major reagents and materials for the cloning, expression and purification of target protein. The target protein is first expressed as a GST fusion protein in E. coli using the GenScript expression vector pGS-21a. The GST fusion protein is bound to a column packed with GenScript Glutathione Resin (L00206) and then eluted with reduced glutathione. Finally, to obtain the tag-free target protein, the purified GST fusion protein is digested with recombinant porcine Enterokinase (EK) to remove GST tag.

Total Volume:

10 ml settled resin (20 ml 50% slurry)

Key Features:

Easy to use: Simple and fast procedures for purification of GST-fusion proteins.
High capacity: The system can support over 20 mg horse liver GST/ml medium.
Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.

Order:

Components:

Components	L00206	L00207	L00208
Glutathione Resin	10 ml (20 ml of 50% slurry)	10 ml (20 ml of 50% slurry )	10 ml (20 ml of 50% slurry )
Columns		5 empty columns	5 empty columns
Glutathione, reduced		5 X 0.154 g	5 X 0.154 g
Enterokinase			2 X 100 IU
Expression Vector			pGS-21a

MSDS:  20050728141208  (PDF)
PROTOCOL:  20051202165130  (PDF)
COA:  L00206_R312904  (PDF)
COA:  L00206_R318905  (PDF)
COA:  L00206_R316904  (PDF)
COA:  L00206_R30121002  (PDF)
COA:  L00206_R30170912  (PDF)
COA:  L00206_R30031002  (PDF)
COA:  L00206_R30221003  (PDF)
COA:  L00206_R60511007  (PDF)
COA:  L00206_R60121009  (PDF)
COA:  L00206_R60401011  (PDF)
COA:  L00206_R60501012  (PDF)
COA:  L00206_R60481103  (PDF)
COA:  L00206_R60071108  (PDF)

Examples:

Fig.1. Purification profile using GenScript Glutathione Resin. Whole proteins (Lane 1) were passed through the resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.

Code	Name	Size	Price
L00207	GST Fusion Protein Purification Kit	1 kit	$ 139.00
L00208	Protein Expression and Purification Kit	1 kit	$ 335.00
A00130	antibody : GST-tag Antibody [HRP], pAb, Rabbit	40 ug	$ 55.00
A00130	antibody : GST-tag Antibody [HRP], pAb, Rabbit	200 ug	$ 248.00
A00097	antibody : GST-tag Antibody, pAb, Rabbit	200 ug	$ 225.00
A00097	antibody : GST-tag Antibody, pAb, Rabbit	40 ug	$ 50.00
M0001	Epitope Tag Positive Control Lysates: GST Lysates	100 ug	$ 72.00


1	Custom Recombinant Protein Services : Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2	Bacterial Expression System: GenScript has developed BacPower^TM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3	Yeast Expression System: GenScript's proprietary YeastHIGH^TM technology combines the advantages of the bacterial and eukaryotic expression systems.
4	Baculovirus/Insect Cell Expression System: GenScript developed BacuVance^TM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5	Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.