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High-Affinity GST Resin

Description:

GenScript High-Affinity GST Resin (L00206) is designed for the rapid, single-step purification of glutathione S-transferases, glutathione-dependent proteins, and recombinant derivatives of glutathione S-transferase, including glutathione S-transferase (GST) fusion proteins expressed in E. coli, insect cells, and mammalian cells. High-Affinity GST Resin can purify GST fusion proteins directly from bacterial lysate using. High-Affinity GST Resin (50% slurry) has a protein binding capacity of over 6 mg GST/ml packed resin.

The GST Fusion Protein Purification Kit (L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript's Protein Expression and Purification Kit (L00208) provides a set of reagents for customers to use to facilitate the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, (His)6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain (EK) is also included in the kit for the cleavage of tags. The recombinant enterokinase (EK) contains a (His)6 tag for easy removal of EK after cleavage using Ni-resin (not included).

Total Volume:

20 ml of 50% slurry

Key Features:

• Easy to perform: GenScript's simple, speedy procedure purifies GST-fusion proteins with minimal hassle.
• High capacity: The system can support over 6 mg horse liver GST/ml medium.
• Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.

Order:

Cat.No. Name Size Price Quantity   L00206 High-Affinity GST Resin  10 ml $ 98.00

Components: Components L00206 L00207 L00208 GST Resin  10 ml (20 ml of 50% slurry)    10 ml (20 ml of 50% slurry )  Columns      5 empty columns  Glutathione, reduced      5 X 0.154 g  Enterokinase      2 X 100 IU  Expression Vector      pGS-21a  Manual  TM0185    TM0186  MSDS:  20050728141208  (PDF) COA:  L00206_R312904  (PDF) COA:  L00206_R318905  (PDF) COA:  L00206_R316904  (PDF) COA:  L00206_R30170912  (PDF) COA:  L00206_R30031002  (PDF) COA:  L00206_R30121002  (PDF) COA:  L00206_R30221003  (PDF) Examples: Fig.1. Purification profile using GenScript High-Affinity GST Resin. Whole proteins (Lane 1) were passed through GST-resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.

Related Products:   Code Name Size Price Quantity L00207  GST Fusion Protein Purification Kit   1 kit  $ 139.00 L00208  Protein Expression and Purification Kit  1 kit  $ 335.00 A00130  antibody : GST-tag Antibody [HRP], pAb, Rabbit  40 ug  $ 55.00 A00130  antibody : GST-tag Antibody [HRP], pAb, Rabbit  200 ug  $ 248.00 A00097  antibody : GST-tag Antibody, pAb, Rabbit  200 ug  $ 225.00 A00097  antibody : GST-tag Antibody, pAb, Rabbit  40 ug  $ 50.00 M0001  Epitope Tag Positive Control Lysates: GST Lysates  100 ug  $ 72.00

1	Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2	Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3	Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
4	Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5	Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.