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High-Affinity GST Resin

Description:


GenScript High-Affinity GST Resin (L00206) is designed for the rapid, single-step purification of glutathione S-transferases, glutathione-dependent proteins, and recombinant derivatives of glutathione S-transferase, including glutathione S-transferase (GST) fusion proteins expressed in E. coli, insect cells, and mammalian cells. High-Affinity GST Resin can purify GST fusion proteins directly from bacterial lysate using. High-Affinity GST Resin (50% slurry) has a protein binding capacity of over 6 mg GST/ml packed resin.

The GST Fusion Protein Purification Kit (L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript's Protein Expression and Purification Kit (L00208) provides a set of reagents for customers to use to facilitate the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, (His)6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain (EK) is also included in the kit for the cleavage of tags. The recombinant enterokinase (EK) contains a (His)6 tag for easy removal of EK after cleavage using Ni-resin (not included).


Key Features:
  • Easy to perform: GenScript's simple, speedy procedure purifies GST-fusion proteins with minimal hassle.
  • High capacity: The system can support over 6 mg horse liver GST/ml medium.
  • Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.
Order:
 Cat.No.NameSizePriceQuantity
 L00206 GST Resin  1 kit$ 98.00


MSDS:  20050728141208  (PDF)
PROTOCOL:  20051202165130  (PDF)


Examples:



Fig.1. Purification profile using GenScript High-Affinity GST Resin. Whole proteins (Lane 1) were passed through GST-resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.










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