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GST Fusion Protein Purification Kit



Description:

GenScript's High-Affinity GST Resin (L00206) is designed for the rapid, single-step purification of glutathione S-transferases, glutathione-dependent proteins, and recombinant derivatives of glutathione S-transferase, including glutathione S-transferase (GST) fusion proteins expressed in E. coli, insect cells, and mammalian cells. High-Affinity GST Resin can purify GST fusion proteins directly from bacterial lysate using. High-Affinity GST Resin (50% slurry) has a protein binding capacity of over 6 mg GST/ml packed resin.

The GST Fusion Protein Purification Kit (L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript's Protein Expression and Purification Kit (L00208) provides a set of reagents for customers to use to facilitate the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, (His)6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain (EK) is also included in the kit for the cleavage of tags. The recombinant enterokinase (EK) contains a (His)6 tag for easy removal of EK after cleavage using Ni-resin (not included).

Key Features:
Order:

 Cat.No.NameSizePriceQuantity
 L00207 GST Fusion Protein Purification Kit   1 kit$ 139.00


Components:

Components L00206L00207L00208
GST Resin 10 ml (20 ml of 50% slurry) 10 ml (20 ml of 50% slurry) 10 ml (20 ml of 50% slurry ) 
Columns  5 empty columns 5 empty columns 
Glutathione, reduced  5 x 0.154 g 5 X 0.154 g 
Enterokinase   2 X 100 IU 
Expression Vector   pGS-21a 
Manual TM0185 TM0185 TM0186 

MSDS:  20081016052705  (PDF)
COA:  GST Fusion Protein Purification Kit  (PDF)


Examples:



Fig.1. Purification profile using GenScript High-Affinity GST Resin. Whole proteins (Lane 1) were passed through GST-resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.










Related Products:


  Code Name Size Price Quantity
L00206  High-Affinity GST Resin  10 ml $ 98.00
L00208  Protein Expression and Purification Kit  1 kit $ 335.00
A00130  antibody : Rabbit Anti-GST-tag [HRP] Polyclonal Antibody  40 ug $ 55.00
A00130  antibody : Rabbit Anti-GST-tag [HRP] Polyclonal Antibody  200 ug $ 247.50
A00097  antibody : Rabbit Anti-GST-tag Polyclonal Antibody  200 ug $ 225.00
A00097  antibody : Rabbit Anti-GST-tag Polyclonal Antibody  40 ug $ 50.00
M0001  Epitope Tag Positive Control Lysates: GST Lysates  100 ug $ 71.50