Protein G Resin


Description:
GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Total Volume:
5 ml settled resin (10 ml 50% slurry)

Key Features:

Characteristics:
LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitution Approximately 2 mg protein G/ml settled resin
Static binding capacity >20 mg sheep IgG/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 12 months when stored unopened at 2-8℃

Order:

 Cat.No.NameSizePriceQuantity
 L00209 Protein G Resin  5 ml$ 129.00

Technical Manual:

EXAMPLE:
Protein G Resin SDS-PAGE
Zoom
PROTOCOL:  20060720235307  (PDF)
COA:  L00209_R313904  (PDF)
COA:  L00209_R321905  (PDF)
MSDS:  20091119194957  (PDF)
COA:  L00209_R317910  (PDF)
COA:  L00209_R30241003  (PDF)
COA:  L00209_R60221009  (PDF)
COA:  L00209_R60431010  (PDF)
COA:  L00209_R60101101  (PDF)
COA:  L00209_R60381103  (PDF)
COA:  L00209_R60591105  (PDF)
COA:  L00209_C40231110  (PDF)



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Z02007  protein : Protein G  5 mg $ 125.00



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