Protein G Resin

Description:

Protein G Resin (Cat. No. L00209) is useful for purification and isolation of IgG. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has three IgG binding domains and other sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant protein G to reduce nonspecific binding. Additionally, 3xCys tag has been attached to the C terminus of recombinant-protein G to facilitate its immobilization. Although the tertiary structures of protein A and protein G are very similar, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G may be used for the purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially certain subclasses including human IgG3, mouse IgG1, and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD, or IgA.

Total Volume:

10 ml of 50% slurry

Key Features:


Components:

LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitutionApproximately 2 mg protein G/ml
Static binding capacity>20 mg sheep IgG/ml drained medium
Stability37°C, 7 days
Matrix sphericalagarose, 4%
Average particle size90 μm (45–165 μm)
SterilizationWash the packed column with 70% ethanol
Storage20% ethanol at +4 to +8°C
Order:

 Cat.No.NameSizePriceQuantity
 L00209 Protein G Resin  5 ml$ 129.00

Technical Manual:

EXAMPLE:
Protein G Resin SDS-PAGE
Zoom
PROTOCOL:  20060720235307  (PDF)
COA:  L00209_R313904  (PDF)
COA:  L00209_R321905  (PDF)
MSDS:  20091119194957  (PDF)
COA:  L00209_R317910  (PDF)



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