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Protein G Resin

Description:

GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Total Volume:

5 ml settled resin (10 ml 50% slurry)

Key Features:

• Broad IgG binding spectrum
• Binding specificity complements of protein A
• Agarose media
• No specific albumin binding
• Optimized homogeneous recombinant ligand
• High capacity
• Available in bulk
• Highly competitive price

Characteristics:

Ligand	Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand	3
MW of ligand	Approximately 22 kDa
PI of ligand	4.69
Degree of substitution	Approximately 2 mg protein G/ml settled resin
Static binding capacity	>20 mg sheep IgG/ml settled resin
Matrix spherical	agarose, 4% cross-linked
Average particle size	90 μm (45-165 μm)
Storage solution	1X PBS containing 20% ethanol
Storage & Stability	12 months when stored unopened at 2-8℃

Order:

Cat.No. Name Size Price Quantity   L00209 Protein G Resin  5 ml $ 129.00

Technical Manual: Download PDF file TM0209 EXAMPLE: Zoom PROTOCOL:  20060720235307  (PDF) COA:  L00209_R313904  (PDF) COA:  L00209_R321905  (PDF) MSDS:  20091119194957  (PDF) COA:  L00209_R317910  (PDF) COA:  L00209_R30241003  (PDF) COA:  L00209_R60221009  (PDF) COA:  L00209_R60431010  (PDF) COA:  L00209_R60101101  (PDF) COA:  L00209_R60381103  (PDF) COA:  L00209_R60591105  (PDF) COA:  L00209_C40231110  (PDF)

Related Products:   Code Name Size Price Quantity Z02007  protein : Protein G  5 mg  $ 125.00

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