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Protein A Resin
Description:
Protein A Resin (Cat. No. L00210) is useful for affinity purification and isolation of IgG. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs, mainly through Fc regions. Native protein A has five IgG binding domains and many other domains of unknown functions. Recombinant protein A contains mainly five high affinity (Ka = 108/M) IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Protein A Resin has been used to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. These qualities of protein A resins combined with GenScript's precision and affordability make GenScript Protein A Resin ideal for the isolation of protein complexes by immunoprecipitation.
Key Features:
- High IgG-binding capacity
- Milder elution condition than protein G resin
- Resin is reusable up to 10 times with no significant loss of binding capacity.
Characteristics:
| Ligand | Recombinant Streptococcal Protein A produced in E. coli |
| Number of IgG binding sites per ligand | 5 |
| MW of ligand | Approximately 43 kDa |
| PI of ligand | 5.17 |
| Degree of substitution | Approximately 2 mg protein A/ml |
| Static binding capacity | >20 mg porcine IgG/ml drained medium |
| Stability | 2-8°C, six months |
| Matrix spherical | agarose, 4% cross-linked |
| Average particle size | 90 μm (45–165 μm) |
| Storage solution | 1× PBS containing 20% ethanol |
| Storage conditions | 2-8°C |
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Technical Manual:
EXAMPLE:  Zoom PROTOCOL: 20060721033243 (PDF) |