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This product has been discontinued!

One-Step Western^TM GST Detection Kit

Kit Description

Based on GenScript's One-Step Western^TM Blot technology, the One-Step Western^TM GST Detection Kit is designed for quick sensitive and reliable detection of GST or GST-fusion proteins by either Western Blot or Dot Blot analysis.

One-Step Western^TM GST Detection Kit contains all the reagents, membrane and HRP substrate necessary to perform a Western or Dot Blot. Neither a primary antibody nor a secondary antibody is needed.

Key features:

• Easy to perform: This kit yields a complete western analysis in under 90 minutes. No secondary antibody is needed.
• Low background: Faster, surer results with journal-quality images.
• High sensitivity: The sensitivity of this kit is comparable to or higher than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
• No optimization is needed.
• Neither a primary antibody nor a secondary antibody is needed.

Applications :

The applications of the One-Step Western^TM GST Detection Kit are as follows:

• Detection of GST and GST-fusion protein
• Confirmation of GST and GST-fusion protein expression
• Screening of GST-fusion protein expression for optimization

Order:

Cat.No. Name Size Price Quantity

Technical Manual: Download PDF file TM0200 Components: Components L00211 Application  Kit for GST detection  Pretreat A solution  100 ml  Pretreat B solution  100 ml  WB solution  100 ml  10X Wash solution  100 ml  Nitrocellulose membrane  10 Sheets  HRP substrate  2 x 30 ml  Protocol  1  Manual  TM0200  PROTOCOL:  20060605155746  (PDF) MSDS:  20060818160706  (PDF) Application Examples: Shown below is a dot blot detection of GST protein. Dot Blot for the detection of GST protein using One-Step WesternTM GST Detection Kit L00211. 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, 2.5 ng and 0.82 ng of GST were spotted on the membrane. The blot was developed with LumiSensor system included in the kit. Quantities of GST as low as 1 ng are cleanly detected. Shown below is a western blot detection of GST protein. Western Blot for the detection of GST protein using One-Step WesternTM GST Detection Kit L00211. 500 ng, 167 ng, 55.6 ng, 18.5 ng, 6.17 ng, 2.05 ng and 0.69 ng of GST were were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6 and Lane 7, respectively. The blot was developed with the LumiSensor system included in the kit.

Related Products:   Code Name Size Price Quantity L00204  ONE-HOUR WesternTM Basic Kit (Rabbit)  1 Kit (10 Assays)  $ 195.00 L00205  ONE-HOUR WesternTM Basic Kit (Mouse)  1 Kit (10 Assays)  $ 195.00 L00204T  ONE-HOUR WesternTM Complete Kit Using TMB (Rabbit)  1 Kit (10 Assays)  $ 255.00 L00205T  ONE-HOUR WesternTM Complete Kit Using TMB (Mouse)  1 Kit (10 Assays)  $ 255.00 L00206  High-Affinity GST Resin  10 ml  $ 98.00 L00224A60  WestClearTM Nitrocellulose Membrane  1 pk (10 sheets of 7.5 x 8 cm)  $ 60.50 L00221V60  LumiSensorTM Chemiluminescent HRP Substrate Kit  1 Kit (2 X 30ml)  $ 55.00