One-Step Western^TM His-Tag Kit

Kit Description

Key Features :

• Easy to perform: This simple and rapid procedure yield a complete Western blot or Dot blot analysis in about one hour.
• High sensitivity and low background: the kit is optimized to give high signal and low background.
• Reproducible results: The kit produces highly reproducible results.
• No optimization is needed.
• Neither a primary antibody nor a secondary antibody is needed.

Applications:

• Detections of His-tagged proteins
• Confirmation of His-tagged protein expression
• Screening of His-tagged protein expression for optimization

Order:

Cat.No. Name Size Price Quantity   L00212 One-Step WesternTM His-Tag Kit  1 kit (10 assays) $ 350.00

Technical Manual: Download PDF file TM0207 Components: Components L00212 Application  Kit for His-tag detection  Pretreat A solution  100 ml  Pretreat B solution  100 ml  WB solution  100 ml  10X Wash solution  100 ml  HRP substrate  2 x 30 ml  Nitrocellulose membrane  10 Sheets  Protocol  1  Manual  TM0207  PROTOCOL:  20060718144859  (PDF) MSDS:  20060718144953  (PDF) Examples: Western Blot detection of His-tagged protein markers. Western Blot for the detection of 6xHis Protein Ladder (Qiagen, Cat. No. 34705) using One-Step WesternTM His-Tag Kit L00212. 10 µl, 5.0 µl, 2.5 µl, 1.3 µl and 0.6 µl of the 6xHis Protein Ladder (reconstituted in 250 µl 1X SDS-PAGE sample buffer as suggested by Qiagen) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, and Lane 5, respectively. The blot was developed with LumiSensor Plus system included in the kit. Picture A above was given an exposure time of 30 seconds and Picture B 60 seconds.