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Note:

This product has been discontinued!

ONE-HOUR Western^TM His-Tag Kit

Kit Description

Based on GenScript ONE-HOUR Western^TM Blot technology, ONE-HOUR Western^TM His-Tag Kit is designed for fast and sensitive detection of His-tagged proteins (including 4xHis-, 5xHis-, and 6xHis-) by either Western blot or Dot blot.

The ONE-HOUR Western^TM His-Tag Kit contains all the necessary reagents, membrane and HRP substrate for Western or Dot blot. Neither a primary antibody nor a secondary antibody is needed.

Key Features :

• Easy to perform: This simple and rapid procedure yield a complete Western blot or Dot blot analysis in about one hour.
• High sensitivity and low background: the kit is optimized to give high signal and low background.
• Reproducible results: The kit produces highly reproducible results.
• No optimization is needed.
• Neither a primary antibody nor a secondary antibody is needed.

Applications:

The application of the ONE-HOUR Western^TM His-Tag Detection Kit are as follows:

• Detections of His-tagged proteins
• Confirmation of His-tagged protein expression
• Screening of His-tagged protein expression for optimization

Order:

Cat.No. Name Size Price Quantity

Technical Manual: Download PDF file TM0207 Components: Components L00212 Application  Kit for His-tag detection  Pretreat A solution  100 ml  Pretreat B solution  100 ml  WB solution  100 ml  10X Wash solution  100 ml  HRP substrate  2 x 30 ml  Nitrocellulose membrane  10 Sheets  Protocol  1  Manual  TM0207  MSDS:  20060818160706  (PDF) PROTOCOL:  20060718144859  (PDF) MSDS:  20060718144953  (PDF) Examples: Western Blot detection of His-tagged protein markers. Western Blot for the detection of 6xHis Protein Ladder (Qiagen, Cat. No. 34705) using ONE-HOUR WesternTM His-Tag Kit L00212. 10 µl, 5.0 µl, 2.5 µl, 1.3 µl and 0.6 µl of the 6xHis Protein Ladder (reconstituted in 250 µl 1X SDS-PAGE sample buffer as suggested by Qiagen) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, and Lane 5, respectively. The blot was developed with LumiSensor Plus system included in the kit. Picture A above was given an exposure time of 30 seconds and Picture B 60 seconds.

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