You are here:   »  One-Step WesternTM Trx Detection Kit

Note:

This product has been discontinued!

One-Step Western^TM Trx Detection Kit

Kit Description:

Based on GenScript One-Step Western^TM Detection technology, the One-Step Western^TM Trx Detection Kit is provides a journal-quality Western blot, revealing Thioredoxin (Trx) protein or proteins tagged with Trx. Using GenScript's breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step Western process, which can take nearly five hours.

The One-Step Western^TM Trx Blot Kit contains all the reagents, membrane, HRP substrate necessary for a Western or Dot blot. Neither a primary antibody nor a secondary antibody is needed.

Key Features:

• Easy to perform: Fewer steps mean fewer chances for human error.
• High sensitivity and low background: The kit reveals even the smallest amounts of relevant protein. It also contains WestClear^TM Nitrocellulose Membrane and LumiSensor^TM Chemiluminescent HRP Substrate Kit, optimized for low background.
• Reproducible results: The kit produces highly reproducible results.
• The kit exhibits excellent linearity.
• The One-Step Western^TM needs far less optimization than the classical three-step method. Neither a primary antibody nor a secondary antibody is needed.

Applications:

The One-Step Western^TM Trx Detection Kit has applications that include the following:

Detection of Trx or Trx-tagged proteins.

Confirmation of Trx-tagged protein expression.

Screening of Trx-tagged protein expression for optimization

Order:

Cat.No. Name Size Price Quantity

Technical Manual: Download PDF file TM0208 Components: Components L00213 Application  Kit for Trx detection  Pretreat A solution  100 ml  Pretreat B solution  100 ml  WB solution  100 ml  10X Wash solution  100 ml  HRP substrate  2 x 30 ml  Nitrocellulose membrane  10 Sheets  Protocol  1  Manual  TM0208  MSDS:  20060718145057  (PDF) PROTOCOL:  20060718145132  (PDF) MSDS:  20060818160706  (PDF) Examples: Western blot detection of Trx protein. Shown below is a western blot detection of Trx protein. Western blot for the detection of recombinant Trx protein using One-Step WesternTM Trx Detection Kit L00213. 50 ng, 25 ng, 12 ng, 6.2 ng, 3.1 ng, 1.6 ng, and 0.8 ng of Trx were loaded in lane 1-7, respectively. The blot was developed with the LumiSensor system included in the kit. A few ng of Trx can be cleanly detected via Western blot using One-Step WesternTM Trx Detection Kit (GenScript, L00213).

1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from . It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.