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One-Step WesternTM Trx Detection Kit


Kit Description:

Based on GenScript One-Step WesternTM Detection technology, the One-Step WesternTM Trx Detection Kit is provides a journal-quality Western blot, revealing Thioredoxin (Trx) protein or proteins tagged with Trx. Using GenScript's breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step Western process, which can take nearly five hours.

The One-Step WesternTM Trx Blot Kit contains all the reagents, membrane, HRP substrate necessary for a Western or Dot blot. Neither a primary antibody nor a secondary antibody is needed.

Key Features:
  • Easy to perform: Fewer steps mean fewer chances for human error.
  • High sensitivity and low background: The kit reveals even the smallest amounts of relevant protein. It also contains WestClearTM Nitrocellulose Membrane and LumiSensorTM Chemiluminescent HRP Substrate Kit, optimized for low background.
  • Reproducible results: The kit produces highly reproducible results.
  • The kit exhibits excellent linearity.
  • The One-Step WesternTM needs far less optimization than the classical three-step method. Neither a primary antibody nor a secondary antibody is needed.

Applications:

The One-Step WesternTM Trx Detection Kit has applications that include the following:
  • Detection of Trx or Trx-tagged proteins.
  • Confirmation of Trx-tagged protein expression.
  • Screening of Trx-tagged protein expression for optimization


    • Order:
     Cat.No.NameSizePriceQuantity
     L00213 One-Step WesternTM Trx Detection Kit  1 kit (10 assays)$ 350.00

    Technical Manual:

    Components:
    ComponentsL00213
    Application Kit for Trx detection 
    Pretreat A solution 100 ml 
    Pretreat B solution 100 ml 
    WB solution 100 ml 
    10X Wash solution 100 ml 
    HRP substrate 2 x 30 ml 
    Nitrocellulose membrane 10 Sheets 
    Protocol 1 
    Manual TM0208 

    MSDS:  20060718145057  (PDF)
    PROTOCOL:  20060718145132  (PDF)

    Examples:

    Western blot detection of Trx protein.
    Shown below is a western blot detection of Trx protein. Western blot for the detection of recombinant Trx protein using One-Step WesternTM Trx Detection Kit L00213. 50 ng, 25 ng, 12 ng, 6.2 ng, 3.1 ng, 1.6 ng, and 0.8 ng of Trx were loaded in lane 1-7, respectively. The blot was developed with the LumiSensor system included in the kit.

    A few ng of Trx can be cleanly detected via Western blot using One-Step WesternTM Trx Detection Kit (GenScript, L00213).

    Examples of using One-step Western<sup>TM</sup> Blot Kit