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One-Step WesternTM HA-Tag Kit


Kit Description

Based on GenScript One-Step WesternTM Blot technology, One-Step WesternTM HA-Tag Kit is designed for fast and sensitive detection of HA-tagged proteins by either Western blot or Dot blot.

One-Step WesternTM HA-Tag Kit contains all the reagents, membrane and HRP substrate for Western or Dot Blot, neither a primary antibody nor a secondary antibody is needed.

Key Features
  • Easy to perform: simple and rapid procedure to perform Western blot, Dot blot in about an hour.
  • High sensitivity and low background: the kit is optimized to give high signal and low background.
  • Reproducible results: the kit produces highly reproducible results.
  • No optimization is needed.
  • Neither a primary antibody nor a secondary antibody is needed.

Applications

One-Step WesternTM HA-Tag Kit enables the fast Western blot or Dot blot for the applications such as:

  • Detecting HA-tagged protein.
  • Checking HA-tagged protein expression.
  • Screening of HA-tagged protein expression for optimization.


Order:
 Cat.No.NameSizePriceQuantity
 L00214 One-Step WesternTM HA-Tag Kit  1 kit (10 assays)$ 350.00

Technical Manual:

Components:
ComponentsL00214
Application HA-tag Detection 
Pretreat A solution 100 ml 
Pretreat B solution 100 ml 
WB Solution 100 ml 
10X Wash solution 100 ml 
HRP substrate 2 x 30 ml 
Nitrocellulose membrane 10 Sheets 
Protocol 1 
Manual TM0211 

PROTOCOL:  20060808135133  (PDF)

Application Examples:

Western blot detection of HA-tagged protein.
Western blot for the detection of HA-tagged protein using One-Step WesternTM HA-Tag Kit L00214. 10 µg, 5.0 µg, 2.5 µg, 1.3 µg, 0.63 µg and 0.31 µg of HA lysate (GenScript, M0004. 293 cells transfected with HA-tagged minigene, 55 kDa) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5 and Lane 6, respectively. The blot was developed with LumiSensorTM system included in the kit. Exposure time was 2 minutes.

Examples of using One-step Western<sup>TM</sup> Blot Kit