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Note:

This product has been discontinued!

One-Step Western^TM DYKDDDDK-Tag Kit

Kit Description

Based on GenScript One-Step Western^TM Blot technology, One-Step Western^TM DYKDDDDK-Tag Kit is designed for fast and sensitive detection of DYKDDDDK-tagged proteins by either Western Blot or Dot Blot.

One-Step Western^TM DYKDDDDK-Tag Kit contains all the reagents, membrane and HRP substrate for Western or Dot Blot, neither a primary antibody nor a secondary antibody is needed.

Key features :

• Easy to perform: simple and rapid procedure to perform Western blot, Dot blot in about an hour.
• High sensitivity and low background: the kit is optimized to give high signal and low background.
• Reproducible results: the kit produces highly reproducible results.
• No optimization is needed.
• Neither a primary antibody nor a secondary antibody is needed.

Applications

One-Step Western^TM DYKDDDDK-Tag Kit enables the fast Western blot or Dot blot for the applications such as:

• Detecting DYKDDDDK-tagged protein.
• Checking DYKDDDDK-tagged protein expression.
• Screening of DYKDDDDK-tagged protein expression for optimization.

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Cat.No. Name Size Price Quantity

Technical Manual: Download PDF file TM0212 Components: Components L00215 Application  Kit for DYKDDDDK-tag detection  Pretreat A solution  100 ml  Pretreat B solution  100 ml  WB solution  100 ml  10X Wash solution  100 ml  HRP substrate  2 x 30 ml  Nitrocellulose membrane  10 Sheets  Protocol  1  Manual  TM0212  PROTOCOL:  20060811144907  (PDF) MSDS:  20060818160706  (PDF) Application Examples: Western blot detection of DYKDDDDK-tagged protein (expressed in E. coli cells). Western Blot for the detection of DYKDDDDK-tagged protein using One-Step WesternTM DYKDDDDK-Tag Kit L00215. 100 ng, 50 ng, 25 ng, 13 ng, 6.3 ng and 3.1 ng of DYKDDDDK-BAP (Sigma, P7582) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5 and Lane 6, respectively. The blot was developed with LumiSensorTM system included in the kit. Exposure time was 2 minutes. Western blot detection of DYKDDDDK-tagged protein (expressed in mammalian cells). Western blot for the detection of DYKDDDDK-tagged protein using One-Step WesternTM DYKDDDDK-Tag Kit L00215. 10 µg, 5.0 µg, 2.5 µg, 1.3 µg and 0.63 µg of DYKDDDDK lysate (GenScript, M0005. 293 cells transfected with DYKDDDDK-tagged minigene, 60 kDa) were loaded in Lane 1, Lane 2, Lane 3, Lane 4 and Lane 5, respectively. The blot was developed with LumiSensor TMsystem included in the kit. Exposure time was 2 minutes.