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Home »  Products »  Immunology Products »  Western Technology »  One-Step Western Kits »  Epitope Tag Detection Kit » One-Step WesternTM DYKDDDDK-Tag Kit

One-Step WesternTM DYKDDDDK-Tag Kit


Kit Description

Based on GenScript One-Step WesternTM Blot technology, One-Step WesternTM DYKDDDDK-Tag Kit is designed for fast and sensitive detection of DYKDDDDK-tagged proteins by either Western Blot or Dot Blot.

One-Step WesternTM DYKDDDDK-Tag Kit contains all the reagents, membrane and HRP substrate for Western or Dot Blot, neither a primary antibody nor a secondary antibody is needed.

Key features :
  • Easy to perform: simple and rapid procedure to perform Western blot, Dot blot in about an hour.
  • High sensitivity and low background: the kit is optimized to give high signal and low background.
  • Reproducible results: the kit produces highly reproducible results.
  • No optimization is needed.
  • Neither a primary antibody nor a secondary antibody is needed.

Applications

One-Step WesternTM DYKDDDDK-Tag Kit enables the fast Western blot or Dot blot for the applications such as:

  • Detecting DYKDDDDK-tagged protein.
  • Checking DYKDDDDK-tagged protein expression.
  • Screening of DYKDDDDK-tagged protein expression for optimization.


Order:
 Cat.No.NameSizePriceQuantity
 L00215 One-Step WesternTM DYKDDDDK-Tag Kit  1 kit (10 assays)$ 350.00

Technical Manual:

Components:
ComponentsL00215
Application Kit for DYKDDDDK-tag detection 
Pretreat A solution 100 ml 
Pretreat B solution 100 ml 
WB solution 100 ml 
10X Wash solution 100 ml 
HRP substrate 2 x 30 ml 
Nitrocellulose membrane 10 Sheets 
Protocol 1 
Manual TM0212 

PROTOCOL:  20060811144907  (PDF)

Application Examples:

Western blot detection of DYKDDDDK-tagged protein (expressed in E. coli cells).
Western Blot for the detection of DYKDDDDK-tagged protein using One-Step WesternTM DYKDDDDK-Tag Kit L00215. 100 ng, 50 ng, 25 ng, 13 ng, 6.3 ng and 3.1 ng of DYKDDDDK-BAP (Sigma, P7582) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5 and Lane 6, respectively. The blot was developed with LumiSensorTM system included in the kit. Exposure time was 2 minutes.



Western blot detection of DYKDDDDK-tagged protein (expressed in mammalian cells).

Western blot for the detection of DYKDDDDK-tagged protein using One-Step WesternTM DYKDDDDK-Tag Kit L00215. 10 µg, 5.0 µg, 2.5 µg, 1.3 µg and 0.63 µg of DYKDDDDK lysate (GenScript, M0005. 293 cells transfected with DYKDDDDK-tagged minigene, 60 kDa) were loaded in Lane 1, Lane 2, Lane 3, Lane 4 and Lane 5, respectively. The blot was developed with LumiSensor TMsystem included in the kit. Exposure time was 2 minutes.