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LumiSensor^TM HRP Substrate Kit

Description

LumiSensor^TM Chemiluminescent HRP Substrate Kit renders horseradish peroxidase (HRP) detectable on immunoblots such as Western and Dot blots. The reagents are optimized for high sensitivity, high intensity, long duration, and low background.

Key Features :

• Easy to perform: The procedure is quick and simple, with only a three-minute incubation period.
• High sensitivity and low background: The kit is optimized to yield clear results each time.
• Reproducible results: The kit produces highly reproducible results.

Order:

Cat.No. Name Size Price Quantity   L00221V60 LumiSensorTM Chemiluminescent HRP Substrate Kit  1 Kit (2 X 30ml) $ 55.00

Technical Manual: Download PDF file TM0201 Components: Components L00225 L00354 L00221V60 L00221V300 L00221V500 Reagent A  30 ml    30 ml  2 X 75 ml  250 ml  Reagent B  30 ml    30 ml  2 X 75 ml  250 ml  Protocol  1    1  1  1  PROTOCOL:  20070410222900  (PDF) Examples: Shown below is a Dot blot detection of IL-8 protein. Figure 1. A Dot blot for IL-8 protein using the One-Step WesternTM Blot Kit (GenScript, L00205) and Mouse IL-8 Antibody (Endogen, M801). 120 ng, 40.0 ng, 13.3 ng, 7.4 ng, 4.4 ng, and 1.5 ng of IL-8 protein were spotted on the membrane. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit. Shown below is a Western blot detection of housekeeping protein α-Tubulin using polyclonal antibody. A Western blot for α-Tubulin using the One-Step WesternTM Blot Kit (GenScript, L00204) and polyclonal α-Tubulin antibody (abcam, ab4074). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg, 0.16 µg and 0.08 µg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit. Shown below is a Western blot detection of housekeeping protein GAPDH using monoclonal antibody. A Western blot for GAPDH using the One-Step WesternTM Blot Kit (GenScript, L00205) and Mouse GAPDH Antibody (abcam, ab8245). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg, 0.16 µg, and 0.08 µg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit.

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