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High Affinity Ni-Charged Resin

Description:

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 ml lab packs.

Total Volume:

10 ml settled resin (20 ml 50% slurry)
25 ml settled resin (50 ml 50% slurry)
500 ml settled resin (1000 ml 50% slurry)

Key Features:

High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/ml (CV).
Simple purification procedure under both native and denaturing conditions.
Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
Resilient: If properly regenerated, the resin can be reused multiple times.

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

Order:

Cat.No.	Name	Size	Price
L00223	High Affinity Ni-Charged Resin	25 ml	$ 168.00
L00223	High Affinity Ni-Charged Resin	10 ml	$ 80.00
L00223	High Affinity Ni-Charged Resin	500 ml	$ 2480.00

PROTOCOL:  20061017234434  (PDF)
COA:  L00223_R301907  (PDF)
COA:  L00223_R319905  (PDF)
COA:  L00223_R30011002  (PDF)
MSDS:  20101019225403  (PDF)
COA:  L00223_R60231008  (PDF)
COA:  L00223_R60121012  (PDF)
COA:  L00223_R60161012  (PDF)
COA:  L00223_R60441011  (PDF)
COA:  L00223_R60131101  (PDF)
COA:  L00223_R60361102  (PDF)
COA:  L00223_R60081104  (PDF)
COA:  L00223_C40251110  (PDF)

Storage:

Store the product between 2�C and 8�C.

Note:

For ambient pressure use only.


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