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High Affinity Ni-Charged Resin

Description:

High Affinity Ni-Charged Resin (Cat. No. L00223) is an agarose resin (4% cross-linked) covalently coupled to a chelating agent that binds Ni²⁺ ions by four coordination sites for high-affinity purification of His-tagged recombinant proteins without leacking of Ni²⁺. His-tagged proteins may be purified under either native or denaturing conditions from any of the common recombinant expression systems, such as bacteria, yeast, insect, and mammalian. Proteins bound to the resin are then eluted with either low pH buffer or imidazole solution or even with histidine solution.

Total Volume:

• 20 ml of 50% slurry
  
• 50 ml of 50% slurry
  
• 1000 ml of 50% slurry

Key Features:

• High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/ml (CV).
• Simple purification procedure under both native and denaturing conditions.
• Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
• Resilient: If properly regenerated, the resin can be reused multiple times.

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

Order:

Cat.No. Name Size Price Quantity   L00223 High Affinity Ni-Charged Resin  25 ml $ 168.00   L00223 High Affinity Ni-Charged Resin  10 ml $ 80.00   L00223 High Affinity Ni-Charged Resin  500 ml $ 2480.00

PROTOCOL:  20061017234434  (PDF) Storage: Store the product between 4°C and 8°C. Note: For ambient pressure use only.