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Note:
This product has been discontinued!

One-Step WesternTM Complete Blot Kit (Chicken)


Kit Description:

One-Step WesternTM Complete Kit (Chicken), based on GenScript's One-Step WesternTM Blot technology, is a complete kit contains all the necessary reagents, buffers, nitrocellulose membrane and HRP substrate for performing Western blot or Dot blot analysis with chicken primary antibody.

The kit contains WestClearTM nitrocellulose membrane (0.2 µm, L00224A60) and a LumiSensorTM Chemiluminescent HRP Substrate Kit (L00221V60) optimized for best results. WestClearTM nitrocellulose membrane and LumiSensorTM Chemiluminescent HRP Substrate Kit are also available separately.

Key Features:

Applications:

The One-Step WesternTM Blot Kit cuts hours off the following applications:



Order:

 Cat.No.NameSizePriceQuantity

Technical Manual:

Components:

Components L00226
Application For Chicken Primary Antibody 
Pretreat A Solution 100 ml 
Pretreat B Solution 100 ml 
WB (C) Solution 100 ml 
10X Wash Solution 100 ml 
Nitrocellulose Membrane 10 sheets 
HRP Substrate 2 X 30 ml 
Protocol 

PROTOCOL:  20060808135336  (PDF)


Examples:

Western blot detection of housekeeping protein β-Actin.
Western blot detection of β-Actin using the One-Step Complete WesternTM Blot Kit (Chicken) (GenScript, L00226) with chicken β-Actin antibody (GeneTex, GTX14001). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg and 0.16 µg of Hela cell lysate (BD Biosciences, 611449) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6 and Lane 7, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.

Examples of using One-step Western<sup>TM</sup> Blot Kit

1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from . It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.