You are here:  Products » Immunoassay Products » Western Technology » ONE-HOUR WesternTM Complete Kit »  One-Step WesternTM Complete Blot Kit (Chicken)

Note:

This product has been discontinued!

One-Step Western^TM Complete Blot Kit (Chicken)

Kit Description:

One-Step Western^TM Complete Kit (Chicken), based on GenScript's One-Step Western^TM Blot technology, is a complete kit contains all the necessary reagents, buffers, nitrocellulose membrane and HRP substrate for performing Western blot or Dot blot analysis with chicken primary antibody.

The kit contains WestClear^TM nitrocellulose membrane (0.2 µm, L00224A60) and a LumiSensor^TM Chemiluminescent HRP Substrate Kit (L00221V60) optimized for best results. WestClear^TM nitrocellulose membrane and LumiSensor^TM Chemiluminescent HRP Substrate Kit are also available separately.

Key Features:

• Easy to perform: Fewer steps mean fewer chances for human error.
• High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
• Reproducible results: The kit produces highly reproducible results.
• Needs far less optimization than the classical three-step method.
• No secondary antibody is needed.

Applications:

The One-Step Western^TM Blot Kit cuts hours off the following applications:

• Protein/antigen detection
• Evaluation of protein expression
• Antibody/antigen titration
• Analysis and comparison of primary antibody activity levels

Order:

Cat.No. Name Size Price Quantity

Technical Manual: Download PDF file TM0206 Components: Components L00226 Application  For Chicken Primary Antibody  Pretreat A Solution  100 ml  Pretreat B Solution  100 ml  WB (C) Solution  100 ml  10X Wash Solution  100 ml  Nitrocellulose Membrane  10 sheets  HRP Substrate  2 X 30 ml  Protocol  1  PROTOCOL:  20060808135336  (PDF) Examples: Western blot detection of housekeeping protein β-Actin. Western blot detection of β-Actin using the One-Step Complete WesternTM Blot Kit (Chicken) (GenScript, L00226) with chicken β-Actin antibody (GeneTex, GTX14001). 10 µg, 5.0 µg, 2.5 µg, 1.25 µg, 0.62 µg, 0.31 µg and 0.16 µg of Hela cell lysate (BD Biosciences, 611449) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6 and Lane 7, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.

1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from . It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.