One-Step Western^TM Complete Blot Kit (Rat)

Kit Description:

Based on GenScript's One-Step Western^TM Blot technology, the One-Step Western^TM Blot Kit reduces the total analysis time for a Western or Dot blot to just one hour. Both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB, are compatible with this kit.

The kit contains WestClear^TM nitrocellulose membrane (0.2 �m, L00224A60) and LumiSensor^TM Chemiluminescent HRP Substrate Kit (L00221V60) optimized for best results. WestClear^TM nitrocellulose membrane and LumiSensor^TM Chemiluminescent HRP Substrate Kit are also available separately.

Key Features:

Easy to perform: Fewer steps mean fewer chances for human error.
High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
Reproducible results: The kit produces highly reproducible results.
Needs far less optimization than the classical three-step method.
No secondary antibody is needed.

Applications:

The One-Step Western^TM Blot Kit cuts hours off the following applications::

Protein/antigen detection
Evaluation of protein expression
Antibody/antigen titration
Analysis and comparison of primary antibody activity levels

Order:

Technical Manual:

Download PDF file TM0214

Components:

Components	L00227
Application	Kit For Rat Primary Antibody
Pretreat A Solution	100 ml
Pretreat B Solution	100 ml
WB (R or M) Solution	100 ml
10X Wash Solution	100 ml
Nitrocellulose Membrane	10 sheets
HRP Substrate	2 X 30 ml
Protocol	1

PROTOCOL: 20060818160513 (PDF)
MSDS: 20060818160706 (PDF)

Examples:

Shown below is a Western blot detection of housekeeping protein α-Tubulin using rat antibody.
Figure 2. Western blot detection of α-Tubulin using One-Step Western^TM Complete Blot Kit (Rat) (GenScript, L00227) and rat α-Tubulin antibody (GeneTex, GTX76511). 10 �g, 5.0 �g, 2.5 �g, 1.25 �g, 0.62 �g, and 0.31 �g of Hela cell lysate (BD Biosciences, 611449) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, and Lane 6, respectively. The blot was developed with the LumiSensor^TM Chemiluminescent HRP Substrate included in the kit.
α-Tubulin from 0.63 �g of Hela cell lysate (Lane 5) can be cleanly detected using the kit.


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from . It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.

One-Step WesternTM Complete Blot Kit (Rat)