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Protein L Resin
Description:
Protein L is an immunoglobulin-binding protein that binds to immunoglobulin kappa light chains without interfering with the antigen-binding site. It binds to a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A and protein G. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L also binds to single-chain variable fragments (Scfv) and Fab fragments.
Protein L Resin is made by covalently coupling purified protein L to an agarose support. This leak-resistant coupling method ensures excellent gel stability and binding characteristics.
Total Volume:
5 ml of 50% slurry
Key Features:
- Broad Ig binding spectrum
- High capacity of more than 15 mg immunoglobulin per ml of resin
- Cross-linked agarose media
Characteristics:
| Ligand | Purified protein L |
| Number of Ig binding sites per ligand | 5 |
| MW of ligand | Approximately 42 KD |
| PI of ligand | 4.57 |
| Degree of substitution | Approximately 2 mg protein L/ml |
| Static binding capacity | >15 mg Porcine Ig/ml drained medium |
| Stability | 37°C, 7 days |
| Matrix spherical | Agarose, 4% cross-linked |
| Average particle size | 90 μm (45–165 μm) |
| Sanitization | Washing of the packed column with 70% ethanol |
| Storage | 20% ethanol at +4 to +8 °C |
Order:
Technical Manual:
EXAMPLE:  Zoom PROTOCOL: 20061219142916 (PDF) COA: L00239_R311904 (PDF) |