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Protein L Resin

Description:

GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg porcine IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein L, first isolated from the surface of bacterial species Peptostreptoccus magnus, binds immunoglobulin through κ light chain interaction. Protein L binds a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments.

Total Volume:

2.5 ml settled resin (5 ml 50% slurry)

Key Features:

• Broad Ig binding spectrum
• High capacity of more than 15 mg immunoglobulin per ml of resin
• Cross-linked agarose media

Characteristics:

Ligand	Highly purified protein L
Number of Ig binding sites per ligand	5
MW of ligand	Approximately 42 KD
PI of ligand	4.57
Degree of substitution	Approximately 2 mg protein L/ml settled resin
Static binding capacity	>15 mg porcine Ig/ml settled resin
Matrix spherical	Agarose, 4% cross-linked
Average particle size	90 μm (45 - 165 μm)
Storage solution	1×PBS containing 20% ethanol
Sanitization	Washing of the packed column with 70% ethanol
Storage & stability	12 months when stored unopened at 2 to 8 °C

Order:

Cat.No. Name Size Price Quantity   L00239 Protein L Resin  2.5 ml $ 145.00

Technical Manual: Download PDF file TM0222 EXAMPLE: Zoom PROTOCOL:  20061219142916  (PDF) COA:  L00239_R311904  (PDF) MSDS:  20090909220137  (PDF) COA:  L00239_R301911  (PDF) COA:  L00239_R30011003  (PDF) COA:  L00239_R30241004  (PDF) COA:  L00239_R60361009  (PDF)

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