Protein L Resin

Description:

Protein L is an immunoglobulin-binding protein that binds to immunoglobulin kappa light chains without interfering with the antigen-binding site. It binds to a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A and protein G. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L also binds to single-chain variable fragments (Scfv) and Fab fragments.
Protein L Resin is made by covalently coupling purified protein L to an agarose support. This leak-resistant coupling method ensures excellent gel stability and binding characteristics.

Total Volume:

5 ml of 50% slurry

Key Features:


Characteristics:

LigandPurified protein L
Number of Ig binding sites per ligand5
MW of ligandApproximately 42 KD
PI of ligand4.57
Degree of substitutionApproximately 2 mg protein L/ml
Static binding capacity>15 mg Porcine Ig/ml drained medium
Stability37°C, 7 days
Matrix sphericalAgarose, 4% cross-linked
Average particle size90 μm (45–165 μm)
SanitizationWashing of the packed column with 70% ethanol
Storage20% ethanol at +4 to +8 °C
Order:

 Cat.No.NameSizePriceQuantity
 L00239 Protein L Resin  2.5 ml$ 145.00

Technical Manual:

EXAMPLE:
SDS-PAGE analysis using Protein L Resin
Zoom
COA:  L00239_R311904  (PDF)
MSDS:  20090909220137  (PDF)
COA:  L00239_R301911  (PDF)
COA:  L00239_R30011003  (PDF)


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