Express^TM ELISA Kit (Mouse)

Description:

The GenScript ExpressTM ELISA Kit is designed to detect and quantitate antigen-specific antibodies in serums and other samples using a proprietary (patent pending) one-hour indirect ELISA procedure. The ExpressTM ELISA kit is ideal for large-scale antibody titer determination. This kit allows user to perform a complete ELISA from antigen coating to signal development in just one hour. The one-hour ExpressTM ELISA procedure is contrasted with a classical five-hour ELISA procedure at right (Figure 1). Coat the plate with an antigen (protein or peptide) in the Coating Buffer for five minutes. Then block the plate with Pretreat Solution for five minutes so that it will be ready for antigen-specific antibody binding. After the 30-minute binding phase, treat the plate with the ELISA solution included in the kit for 20 minutes. The plate can then be developed with the One-Solution Microwell TMB solution included in the kit. The kit contains all necessary reagents and buffers for performing indirect ELISA using ten 96-well plates.

Key Features:

• Quick: This kit cuts indirect ELISA time down to one hour.
• High sensitivity: The kit's sensitivity is the same as or better than that of the classical procedure.
• Reproducible results: The kit produces highly reproducible results.
• Less optimization: The Express^TM ELISA Kit needs far less optimization than the classical ELISA method.

Storage:

Order:

Cat.No. Name Size Price Quantity   L00252 ExpressTM ELISA Kit (Mouse)  1 Kit $ 396.00

Technical Manual: Download PDF file TM0239 Components: Components L00251 L00252 L00253 Application  Rabbit Primary Antibody  Mouse Primary Antibody  Goat Primary Antibody  2X Coating Buffer  125 ml  125 ml  125 ml  Pretreat A Solution  125 ml  125 ml  125 ml  Pretreat B Solution  125 ml  125 ml  125 ml  Dilution Solution  125 ml  125 ml  125 ml  ELISA Solution  125 ml  125 ml  125 ml  20X Wash Solution  125 ml  125 ml  125 ml  One-Solution Microwell TMB  110 ml  110 ml  110 ml  Stop Solution  125 ml  125 ml  125 ml  Protocol  1  1  1  Manual  TM0239  TM0239  TM0239  EXAMPLE: Zoom PROTOCOL:  20070510052031  (PDF)

Related Products:   Code Name Size Price Quantity M00078  Immunology Reagents: One-Solution Microwell TMB  110 ml  $ 39.50 M01016  Solution: 20X Wash Solution  125 ml  $ 69.00 M01017  Solution: Stop Solution  125 ml  $ 24.50 M01018  Buffer: 2X Coating Buffer  125 ml  $ 39.50 M01019  Solution: Pretreat Solution (A + B)  250 ml  $ 149.00