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Note:
This product has been discontinued!
The improved replacement is  L00411

ONE-HOUR ELISATM GST Detection Kit



Description:

The GenScript ONE-HOUR ELISATM GST Detection Kit allows the use to detect and quantitate glutathione-S-transferase (GST) and GST-fusion proteins in samples, such as cell lysate, in just 35 minutes. GenScript's ONE-HOUR ELISATM procedure is contrasted with the classical five-hour Sandwich ELISA procedure at right (Figure 1).

Incubate the pre-coated and pre-blocked plate with the sample and the ELISA solution included in the kit for 20 minutes. Then wash the plates five times, and it will be ready for development with the One-Solution Microwell TMB solution included in the kit.

The kit contains all reagents and buffers necessary for performing ELISA using five 96-well plates. No secondary antibody is needed.

Key Features:
  • Quick: This kit allows user to quantitate GST or GST-fusion protein in just 35 minutes.
  • Reproducible results: The kit produces highly reproducible results.


Applications:
  • Detection of GST or GST-fusion proteins
  • Confirmation of GST or GST-fusion protein expression
  • Screening of GST-fusion protein expression for expression optimization




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 Cat.No.NameSizePriceQuantity

Technical Manual:

Components:

Components L00234L00236L00244L00246L00247L00248L00249L00254L00255L00285L00292L00325L00326L00352L00416
Pre-coated plate (12 strips per plate)   200 μl     5        
Dilution solution   20 μl     100 ml        
GST ELISA solution        30 ml        
GST standard protein (0.7 mg/ml)        40 μl        
20X Wash solution        55 ml        
One-Solution Microwell TMB        55 ml        
Stop solution        55 ml        
Protocol        1        
Manual        TM0240        


Storage:

Store the kit at 4°C. It will remain stable for six months. Do not freeze the kit or any of its components.

Related Products:

  Code Name Size Price Quantity
M00078  Immunology Reagents: One-Solution Microwell TMB  110 ml $ 40.00
M01016  Solution: 20X Wash Solution  125 ml $ 69.00
M01017  Solution: Stop Solution  125 ml $ 24.00
Z02039  protein : Glutathione S-Transferase (GST)， Schistosoma japonicum  1 mg $ 70.00
A00097  antibody : GST-tag Antibody, pAb, Rabbit  200 ug $ 225.00
A00097  antibody : GST-tag Antibody, pAb, Rabbit  40 ug $ 50.00
A00130  antibody : GST-tag Antibody [HRP], pAb, Rabbit  40 ug $ 55.00
A00130  antibody : GST-tag Antibody [HRP], pAb, Rabbit  200 ug $ 248.00



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