The GenScript One-Step ELISATM GST Detection Kit allows the use to detect and quantitate glutathione-S-transferase (GST) and GST-fusion proteins in samples, such as cell lysate, in just 35 minutes.GenScript's One-Step ELISATM procedure is contrasted with the classical five-hour Sandwich ELISA procedure at right (Figure 1).
Incubate the pre-coated and pre-blocked plate with the sample and the ELISA solution included in the kit for 20 minutes. Then wash the plates five times, and it will be ready for development with the One-Solution Microwell TMB solution included in the kit.
The kit contains all reagents and buffers necessary for performing ELISA using ten 96-well plates. No secondary antibody is needed.
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