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ONE-HOUR ELISATM GST Detection Kit



Description:

The GenScript ONE-HOUR ELISATM GST Detection Kit allows the use to detect and quantitate glutathione-S-transferase (GST) and GST-fusion proteins in samples, such as cell lysate, in just 35 minutes. GenScript's ONE-HOUR ELISATM procedure is contrasted with the classical five-hour Sandwich ELISA procedure at right (Figure 1).

Incubate the pre-coated and pre-blocked plate with the sample and the ELISA solution included in the kit for 20 minutes. Then wash the plates five times, and it will be ready for development with the One-Solution Microwell TMB solution included in the kit.

The kit contains all reagents and buffers necessary for performing ELISA using five 96-well plates. No secondary antibody is needed.

Key Features:
  • Quick: This kit allows user to quantitate GST or GST-fusion protein in just 35 minutes.
  • Reproducible results: The kit produces highly reproducible results.


Applications:
  • Detection of GST or GST-fusion proteins
  • Confirmation of GST or GST-fusion protein expression
  • Screening of GST-fusion protein expression for expression optimization




Order:

 Cat.No.NameSizePriceQuantity
 L00254 ONE-HOUR ELISATM GST Detection Kit  1 Kit$ 298.00

Technical Manual:

Components:

Components L00254
Pre-coated plate (12 strips per plate) 5 
Dilution solution 100 ml 
GST ELISA solution 30 ml 
GST standard protein (0.7 mg/ml) 40 µl 
20X Wash solution 55 ml 
One-Solution Microwell TMB 55 ml 
Stop solution 55 ml 
Protocol 1 
Manual TM0240 


Storage:

Store the kit at 4°C. It will remain stable for six months. Do not freeze the kit or any of its components.

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M01017  Solution: Stop Solution  125 ml $ 24.50
Z02039  protein : Glutathione S-Transferase (GST)， Schistosoma japonicum  1 mg $ 70.00
A00097  antibody : Rabbit Anti-GST-tag Polyclonal Antibody  200 ug $ 225.00
A00097  antibody : Rabbit Anti-GST-tag Polyclonal Antibody  40 ug $ 50.00
A00130  antibody : Rabbit Anti-GST-tag [HRP] Polyclonal Antibody  40 ug $ 55.00
A00130  antibody : Rabbit Anti-GST-tag [HRP] Polyclonal Antibody  200 ug $ 247.50