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IminoBiotin Resin
Kit Description:
Genscript IminoBiotin Resin is for the purification of avidin, streptavidin or their conjugates. Iminobiotin is the cyclic guanido analog of biotin that has a lower affinity constant for binding avidin, streptavidin. The Iminobiotin analog can be used in situations requiring mild dissociation of the avidin-biotin complex. Normally, disrupting an avidin-biotin interaction requires 6-8 M guanidine•HCl, pH 1.5, an environment that is often too harsh for proteins to maintain native structure or activity. Iminobiotin binds at pH values above 9.5 and elution is performed at pH 4.0.
Key Features:
- High capacity: > 20 mg Streptavidin (GenScript, Cat. No. Z02043) per ml settled resin
- Reusable: 5-10 times can be reused with no decrease of binding capacity.
- Agarose media: 4% beaded agarose
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