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Protein A MagBeads


Kit Description:
Protein A MagBeads (GenScript, L00273) is BioMag particles of approximately 20-75 μm in size, covalently coated with Protein A. The particles are held in a suspension supplied in 1×PBS with 20% ethanol added. BioMag Protein A is ready to use. BioMag Protein A is suitable for use in binding immunoglobulins, as an effective adsorbant for removal of Fc fragments during Fab fragment preparation, in immunoassay systems, and it is ready to use after one quick vortex or even a vigorous shake.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs, mainly through Fc regions. Native Protein A has five IgG binding domains and many unknown-function repeated sequences. Recombinant Protein A contains five high-affinity (Ka=108/M) IgG binding domains to reduce nonspecific binding.
Key Features:

  • Quick and convenient separation accomplished by magnetic means
  • High capacity: The product can manage over 10 mg/ml porcine IgG
  • Low rate of nonspecific binding
  • Optimized homogeneous recombinant ligands
    Order:

     Cat.No.NameSizePriceQuantity
     L00273 Protein A MagBeads  1 ml$ 89.00


    Technical Manual:

    EXAMPLE:
    Protein A MagBeads
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    PROTOCOL:  20070807043251  (PDF)
    COA:  L00273_R315908  (PDF)
    COA:  L00273_R322906  (PDF)


    Related Products:

      Code Name Size Price Quantity
    L00209  Protein G Resin  5 ml $ 129.00
    L00210  Protein A Resin  5 ml $ 98.00
    L00239  Protein L Resin  2.5 ml $ 145.00



    1
    		 Custom Recombinant Protein Services : Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    		 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    		 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    		 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    		 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.