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Protein G MagBeads

Description:

Protein G MagBeads (GenScript, L00274) is a suspension of magnetic particles with the size of approximately 20-75 μm that are covalently coupled with Protein G. The suspension is supplied in 2 ml of 1XPBS containing 20% ethanol with 0.5 ml of MagBeads (bed volume). BioMag Protein G is suitable for binding and purification of immunoglobulins. It can be used for immunoprecipitation (IP), it can also be used for the removal of Fc fragments during Fab fragment preparation. Quick and convenient separations are accomplished magnetically.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Key Features:

  • Quick and convenient separation accomplished by magnetic forces
  • High capacity: 0.5 mL Protein G MagBeads (bed volume) can bind more than 5 mg of goat IgG
  • Low nonspecific binding
    • Order:

     Cat.No.NameSizePriceQuantity
     L00274 Protein G MagBeads  0.5 ml$ 125.00


    Technical Manual:

    EXAMPLE:
    Protein G MagBeads
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    DATASHEET:  20070917044631  (DOC)
    DATASHEET:  20070917044758  (PDF)