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Protein G MagBeads

Description:

Protein G MagBeads (GenScript, L00274) is a suspension of magnetic particles with the size of approximately 20-75 μm that are covalently coupled with Protein G. The suspension is supplied in 2 ml of 1XPBS containing 20% ethanol with 0.5 ml of MagBeads (bed volume). BioMag Protein G is suitable for binding and purification of immunoglobulins. It can be used for immunoprecipitation (IP), it can also be used for the removal of Fc fragments during Fab fragment preparation. Quick and convenient separations are accomplished magnetically.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Key Features:

  • Quick and convenient separation accomplished by magnetic forces
  • High capacity: 0.5 mL Protein G MagBeads (bed volume) can bind more than 5 mg of goat IgG
  • Low nonspecific binding
    • Order:

     Cat.No.NameSizePriceQuantity
     L00274 Protein G MagBeads  0.5 ml$ 125.00


    Technical Manual:

    EXAMPLE:
    Protein G MagBeads
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    DATASHEET:  20070917044631  (DOC)
    DATASHEET:  20070917044758  (PDF)

    1
    		 Custom Recombinant Protein Services : Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    		 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    		 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    		 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    		 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.