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Protein A/G MagBeads
Description:
Protein A/G MagBeads (GenScript, L00277) are BioMag particles of approximately 20-75 μm in size that are covalently coated with Protein A/G. The product is supplied as suspension of 0.5 ml of MagBeads (bed volume) in 2 ml of 1×PBS containing 20% ethanol. BioMag Protein A/G is an excellent purification tool for most immunoglobulins. Protein A/G binds to all human IgG subclasses and also binds somewhat to IgA, IgE, IgM and, to a lesser extent, IgD. Therefore, it has a much broader binding range than either Protein A or Protein G individually. Unlike non-recombinant Protein G, Protein A/G does not bind serum albumin because the gene sequence coding for the albumin-binding site has been eliminated. Protein A/G is effective for mouse monoclonal antibody purification from IgG subclasses because Protein A/G binds all mouse IgG subclasses but does not bind murine IgA, IgM or serum albumin. It is ready to use after a gentle shake.
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.
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