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Annexin V-EGFP Apoptosis Detection Kit

Description:

The Annexin V-EGFP Apoptosis Detection Kit (Cat. No. L00288) is a new product from GenScript. Most of the phosphatidylserines (PS) in cell membrane phospholipids translocate from the inner surface to the outer surface during the early stages of apoptosis. Once the PS are on the outer surface, they can be detected easily by staining then with an enhanced green fluorescent protein (EGFP) fused with annexin V, a protein that has strong natural affinity with PS. In addition, propidium iodide (PI) is a nucleic dye. It cannot be through cellular membrane directly, but it can pass through the membranes of cells in middle and late period of apoptosis and stain their nuclei red. Detection can be analyzed by flow cytometry and fluorescence microscopy with a FITC filter. EGFP is brighter and much more photo-stable than other fluorescent reagents, such as FITC.

Key Features:

  • Simplified Procedure: This kit is precise and easy to use.
  • High Precision: This kit can detect cells in the early period of apoptosis and accurately differentiate and compute the ratio of normal cells, apoptosis cells, and necrotic cells.
  • High Stabilization: EGFP has strong signals that are hard to quench.

    Applications:

    For the detection of apoptotic cells by flow cytometry and Fluorescence Microscopy.
    Order:

     Cat.No.NameSizePriceQuantity
     L00288 Annexin V-EGFP Apoptosis Detection Kit  1 kit$ 168.00

    Technical Manual:


    Components:

    Components
    Annexin V-EGFP 
    Binding Buffer 
    Propidium Iodide （PI） 

    EXAMPLE:
    Annexin V-EGFP Apoptosis Detection Kit
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