You are here:  Products » Cell Biology Products » Apoptosis Detection Kits »  Caspase-3 Colorimetric Assay Kit

Caspase-3 Colorimetric Assay Kit

Description

The Caspase-3 Colorimetric Assay Kit (Cat. No. L00289) provides a simple and convenient means of assaying the activity of caspase. Caspase (Cysteine-requiring Aspartate Protease) is a protease family, and it plays a very important role in apoptosis. This kit is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA can be quantified using a spectrophotometer or a microtiter plate reader at 400 nm or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-3 activity can be determined.

Key features:

Simplified Procedure: The components in this kit are provided ready-to-use.

Supreme Reliability: The kit produces highly reproducible results.

Order:

Cat.No. Name Size Price Quantity   L00289 Caspase-3 Colorimetric Assay Kit  1 kit $ 275.00

Components: Components L00289 L00294 L00302 L00303 L00304 Lysis Buffer  15.0 ml  15.0 ml  15.0 ml  15.0 ml  15.0 ml  2X Reaction Buffer  5.0 ml  5.0 ml  5.0 ml  5.0 ml  5.0 ml  Substrate  500 µl, store away from light  500 µl, store away from light  500 µl, storeaway from light  500 µl, storeaway from light  500 µl, store away from light  DTT  150 µl  150 µl  150 µl  150 µl  150 µl  PROTOCOL:  20080219214753  (PDF)

Related Products:   Code Name Size Price Quantity L00294  Caspase-2 Colorimetric Assay Kit  1 kit  $ 275.00 L00302  Caspase-6 Colorimetric Assay Kit  1 kit  $ 275.00 L00303  Caspase-8 Colorimetric Assay Kit  1 kit  $ 275.00 L00304  Caspase-9 Colorimetric Assay Kit  1 kit  $ 275.00