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TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)

Description

The TUNEL Universal Apoptosis Detection Kit (Biotin-labeled POD) (Cat. No. L00290) is one of GenScript’s newly introduced products. It is used for assaying adherent Cells, paraffin-embedded tissue sections and cryopreserved tissue sections. The Kit could detect the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-Diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key features

Simplified Procedure: The kit contains ready-to-use reagents, including Proteinase K, DAB-Solution and DNase I.

Enhanced Sensitivity: The kit can assay the cells in early period of apoptosis.

Enhanced Specificity: The kit can stain apoptosis cells.

Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.

Streamlined Process: The whole process takes about three hours.

Increased Convenience: The result can be observed by light microscope.

High Veracity: The kit contains positive control reagent.

Order:

Cat.No. Name Size Price Quantity   L00290 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  100 Assays $ 598.00   L00290 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  20 Assays $ 246.00   L00290 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  50 Assays $ 437.00

Technical Manual: Download PDF file TM0267 Components: Components L00290 Equilibration Buffer  5 ml (for 100 assays)  Biotin-11-dUTP  100 μl (for 100 assays)  TdT  400 μl (for 100 assays)  50X Proteinase K (1mg/ml)  200 μl (for 100 assays)  Streptavidin-HRP  50 μl (for 100 assays)  DAB-A Solution  600μL (for 100 assays)  DAB-B Solution  600μL (for 100 assays)  DAB-C Solution  600μL (for 100 assays)  DNase I (50 U/μl)  1ml (for 100 assays)  DNase I buffer  1ml (for 100 assays)  PROTOCOL:  20080611021337  (PDF) MSDS:  20090218071040  (PDF)

Related Products:   Code Name Size Price Quantity L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  100 Assays  $ 598.00 L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  20 Assays  $ 238.00 L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  50 Assays  $ 421.00 L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  100 Assays  $ 598.00 L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  20 Assays  $ 238.00 L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  50 Assays  $ 421.00 L00298  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Sections, Biotin labeled POD)  100 Assays  $ 598.00 L00299  TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  100 Assays  $ 598.00 L00299  TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  20 Assays  $ 254.00 L00299  TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  50 Assays  $ 445.00 L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  100 Assays  $ 598.00 L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  20 Assays  $ 254.00 L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  50 Assays  $ 445.00 L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  100 Assays  $ 598.00 L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  20 Assays  $ 254.00 L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  50 Assays  $ 445.00

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