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Ni-Charged Magnetic Beads

Description:

GenScript's Nickel-Charged MagBeads (Cat. No. L00295) are magnetic beads with an average diameter of 40-µm pre-charged with Ni2+. They are ideal for rapid purification of 6xHis-tagged target proteins and require minimal handling. Ni-charged MagBeads have a binding capacity of 5 - 20 mg of 6xHis-tagged target protein per 1 ml of beads (CV). The beads are compatible to magnetic-separation-based high-throughput applications. Proteins can be purified under non-denaturing conditions or in the presence of 8 M urea.

Key Features:

  • Quick and convenient separation accomplished by magnetic force
  • High binding capacity: 5 - 20 mg of 6xHis-tagged protein/ml (CV)
  • Compatible with various reagents needed in the purification process,see table 1
  • Resin can be regenerated for multiple uses
  • Low nonspecific binding
    Table 1 Reagents Compatible with Ni-charged Magnetic Beads

    Denaturants Detergents Reducing agents Others Salts
    6 M Gu•HCl 2% Triton X-100 20 mM β-ME 50% glycerol 4 M MgCl2
    8 M Urea 2% Tween 20 1 mM DTT 20% ethanol 5 mM CaCl2
    1% CHAPS 1 mM EDTA 2 M NaCl
    Order:

     Cat.No.NameSizePriceQuantity
     L00295 Ni-Charged Magnetic Beads  2 ml$ 98.00


    Technical Manual:

    EXAMPLE:
    purification of a His-tagged protein using GenScript Ni-Charged MagBeads (Cat. No. L00295)
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