Proteins, Expression, Isolation and Analysis
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Ni-charged MagBeads

Description:

The GenScript Ni-charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.

The GenScript Ni-Charged MagBeads are average 40µm in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6×His-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).

Key Features:

  • Quick and convenient separation accomplished by magnetic force
  • High binding capacity with 5 - 20 mg of 6xHis-tagged protein/ml
  • Compatible with various reagents needed in the purification process(see table 1)
  • Resin can be regenerated for multiple uses
  • Extremely low nonspecific binding

    Table 1 Reagents Compatible with Ni-charged Magnetic Beads

    Denaturants Detergents Reducing agents Salts Others
    6 M Gu•HCl 2% Triton X-100 20 mM β-ME 4 M MgCl2 50% glycerol
    8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl2 20% ethanol
    1% CHAPS 2 M NaCl 1 mM EDTA
    Order:

     Cat.No.NameSizePriceQuantity
     L00295 Ni-charged MagBeads  8 ml$ 98.00


    EXAMPLE:
    purification of a His-tagged protein using GenScript Ni-Charged MagBeads (Cat. No. L00295)
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    PROTOCOL:  20100822062936  (PDF)

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