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TUNEL Apoptosis Detection Kit (Adherent Cell, Biotin-labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (Biotin-labeled POD) (Cat. No.L00296) is one of GenScript’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3,3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
    Order:

     Cat.No.NameSizePriceQuantity
     L00296 TUNEL Apoptosis Detection Kit (Adherent Cell, Biotin-labeled POD)  1 kit$ 598.00

    Technical Manual:


    Components:
    ComponentsL00290L00296L00297L00298L00299L00300L00301
    Equilibration Buffer 5.0 ml 5.0 ml 5.0 ml 5.0 ml 5.0 ml 5.0 ml 5.0 ml 
    Biotin-11-dUTP 100 µl 100 µl 100 µl 100 µl    
    FITC-12-dUTP     100 µl 100 µl 100 µl 
    TdT 400 µl 400 µl 400 µl 400 µl 400 µl 400 µl 400 µl 
    50X Proteinase K 200 µl  200 µl   200 µl  
    Streptavidin-HRP 50 µl 50 µl 50 µl 50 µl    
    Anti-fluorescein Antibody     1000 µl 1000 µl 1000 µl 
    DAB 10 mg 10 mg 10 mg 10 mg 10 mg 10 mg 10 mg 

    PROTOCOL:  20080201044510  (PDF)


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