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TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
    Order:

     Cat.No.NameSizePriceQuantity
     L00297 TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  1 kit$ 598.00


    Components:
    Components L00290L00296L00297L00298L00299L00300L00301
    Equilibration Buffer 5.0 ml 5.0 ml 5.0 ml  5.0 ml 5.0 ml 5.0 ml 
    Biotin-11-dUTP 100 µl 100 µl 100 µl     
    FITC-12-dUTP     100 µl 100 µl 100 µl 
    TdT 400 µl 400 µl 400 µl  400 µl 400 µl 400 µl 
    50X Proteinase K 200 µl  200 µl   200 µl  
    Streptavidin-HRP 50 µl 50 µl 50 µl     
    Anti-fluorescein Antibody     1000 µl 1000 µl 1000 µl 
    DAB 10 mg 10 mg 10 mg  10 mg 10 mg 10 mg 

    PROTOCOL:  20080201042913  (PDF)


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    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD) 1 kit 
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    L00296  TUNEL Apoptosis Detection Kit (Adherent Cell, Biotin-labeled POD) 1 kit 
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    L00298  TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD) 1 kit 
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    L00299  TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD) 1 kit 
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    L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC labeled POD) 1 kit 
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    L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD) 1 kit 
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