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TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD)
TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD ) (Cat. No. L00298) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.Key features
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PROTOCOL: 20080201044235 (PDF) |








