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TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD)

TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD ) (Cat. No. L00298) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: TThe entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
    Order:

     Cat.No.NameSizePriceQuantity
     L00298 TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD)  1 kit$ 598.00

    Technical Manual:


    PROTOCOL:  20080201044235  (PDF)



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