Cellular Analysis
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TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (FITC-labeled POD) (Cat. No. L00299) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody conjugated Peroxidase (POD) is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution,a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution, and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by fluorescence microscope and light microscope.
    Order:

     Cat.No.NameSizePriceQuantity
     L00299 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  100 Assays$ 598.00
     L00299 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  20 Assays$ 254.00
     L00299 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  50 Assays$ 445.00

    Technical Manual:

    Components:

    Components L00299
    Equilibration Buffer 5.0 ml (for 100 Assays) 
    FITC-12-dUTP 100 μl (for 100 Assays) 
    TdT 400 μl (for 100 Assays) 
    POD-conjugated Anti-FITC antibody 1000 μl (for 100 Assays) 
    DAB-A Solution 600μL (for 100 Assays) 
    DAB-B Solution 600μL (for 100 Assays) 
    DAB-C Solution 600μL (for 100 Assays) 
    DNase I (50 U/µl) 1ml (for 100 Assays) 
    DNase I buffer 1ml (for 100 Assays) 

    PROTOCOL:  20080201044620  (PDF)
    MSDS:  20090218074939  (PDF)


    Related Products:

      Code Name Size Price Quantity
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  100 Assays $ 598.00
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  20 Assays $ 246.00
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  50 Assays $ 437.00
    L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  100 Assays $ 598.00
    L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  20 Assays $ 238.00
    L00296  TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)  50 Assays $ 421.00
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  100 Assays $ 598.00
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  20 Assays $ 246.00
    L00290  TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)  50 Assays $ 437.00
    L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  100 Assays $ 598.00
    L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  20 Assays $ 238.00
    L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)  50 Assays $ 421.00
    L00298  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Sections, Biotin labeled POD)  100 Assays $ 598.00
    L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  100 Assays $ 598.00
    L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  20 Assays $ 254.00
    L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)  50 Assays $ 445.00
    L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  100 Assays $ 598.00
    L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  20 Assays $ 254.00
    L00301  TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  50 Assays $ 445.00



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