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TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (FITC labeled POD) (Cat. No. L00299) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody and HRP is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by fluorescence microscope and light microscope.
    Order:

     Cat.No.NameSizePriceQuantity
     L00299 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)  1 kit$ 598.00

    Technical Manual:

    Components:

    Components L00290L00296L00297L00298L00299L00300L00301
    Equilibration Buffer 5.0 ml 5.0 ml 5.0 ml  5.0 ml 5.0 ml 5.0 ml 
    Biotin-11-dUTP 100 µl 100 µl 100 µl     
    FITC-12-dUTP     100 µl 100 µl 100 µl 
    TdT 400 µl 400 µl 400 µl  400 µl 400 µl 400 µl 
    50X Proteinase K 200 µl  200 µl   200 µl  
    Streptavidin-HRP 50 µl 50 µl 50 µl     
    HPR-labeled Anti-FITC Antibody     1000 µl 1000 µl 1000 µl 
    DAB 10 mg 10 mg 10 mg  10 mg 10 mg 10 mg 

    PROTOCOL:  20080201044620  (PDF)


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