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TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)

Description

TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (FITC-labeled POD) (Cat. No.L00301) is used for the detection of fragmented DNA in the nucleus duringapoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides binds with the DNA 3´-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observe by fluorescence microscope. And then the compound of anti-fluorescein antibody and HRP is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3,3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.


Key Features

  • Simplified Procedure: The kit contains ready-to-use reagents, including DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by fluorescence microscope and light microscope

    Order:

     Cat.No.NameSizePriceQuantity
     L00301 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  1 kit$ 598.00

    Technical Manual:


    Components:
    Components L00290L00296L00297L00298L00299L00300L00301
    Equilibration Buffer 5.0 ml 5.0 ml 5.0 ml  5.0 ml 5.0 ml 5.0 ml 
    Biotin-11-dUTP 100 µl 100 µl 100 µl     
    FITC-12-dUTP     100 µl 100 µl 100 µl 
    TdT 400 µl 400 µl 400 µl  400 µl 400 µl 400 µl 
    50×Proteinase K 200 µl  200 µl   200 µl  
    Streptavidin-HRP 50 µl 50 µl 50 µl     
    HPR-labeled Anti-FITC Antibody     1000 µl 1000 µl 1000 µl 
    DAB 10 mg 10 mg 10 mg  10 mg 10 mg 10 mg 

    PROTOCOL:  20080226040321  (PDF)


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    L00296  TUNEL Apoptosis Detection Kit (Adherent Cell, Biotin-labeled POD) 1 kit 
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    L00297  TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD) 1 kit 
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    L00298  TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD) 1 kit 
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    L00299  TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD) 1 kit 
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    L00300  TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC labeled POD) 1 kit 
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