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TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)

Description

TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (FITC-labeled POD) (Cat. No.L00301) is used for the detection of fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides binds with the DNA 3´-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody conjugated Peroxidase (POD) is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-Diaminobenzidine (DAB) -Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key Features

Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution and DNase I.

Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.

Enhanced Specificity: The kit can stain apoptotic cells.

Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.

Streamlined Process: The entire procedure takes about three hours.

Increased Convenience: The result can be observed by fluorescence microscope and light microscope.

High Veracity: The kit contains positive control reagent.

Order:

Cat.No. Name Size Price Quantity   L00301 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  100 Assays $ 598.00   L00301 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  20 Assays $ 254.00   L00301 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)  50 Assays $ 445.00

Technical Manual: Download PDF file TM0269 Components: Components L00301 Equilibration Buffer  5.0 ml (for 100 assays)  FITC-12-dUTP  100 μl (for 100 assays)  TdT  400 μl (for 100 assays)  POD-conjugated Anti-FITC antibody  1000 μl (for 100 assays)  DAB-A Solution  600μL (for 100 assays)  DAB-B Solution  600μL (for 100 assays)  DAB-C Solution  600μL (for 100 assays)  DNase I (50 U/µl)  1ml (for 100 assays)  1X DNase I buffer  1ml (for 100 assays)  PROTOCOL:  20080226040321  (PDF) MSDS:  20090218075121  (PDF)

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