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Caspase-8 Colorimetric Assay Kit

Description:

The Caspase-8 Colorimetric Protease Assay Kit (Cat. No. L00303) provides a simple and convenient means of assaying the activity of caspase-8. The activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells. The assay for caspase-8 is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-8 activity can be determined.
Key features:

  • Simplified Procedure: The components in this kit are provided ready-to-use.
  • Supreme Reliability: The kit produces highly reproducible results.
    Order:

     Cat.No.NameSizePriceQuantity
     L00303 Caspase-8 Colorimetric Assay Kit  1 kit$ 275.00


    Components:
    ComponentsL00289L00294L00302L00303L00304
    Lysis Buffer 15.0 ml 15.0 ml 15.0 ml 15.0 ml 15.0 ml 
    2X Reaction Buffer 5.0 ml 5.0 ml 5.0 ml 5.0 ml 5.0 ml 
    Substrate 500 µl, store away from light 500 µl, store away from light 500 µl, storeaway from light 500 µl, storeaway from light 500 µl, store away from light 
    DTT 150 µl 150 µl 150 µl 150 µl 150 µl 

    PROTOCOL:  20080220001412  (PDF)


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