PCR and Cloning
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CloneEZ® PCR Cloning Kit


Description
The GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. Using our proprietary CloneEZ® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C).

Key Features
The CloneEZ® PCR Cloning Kit offers flexible and quick directional cloning and enables users to::
  • Clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases
  • Provide up to 12 kb PCR cloning at any restriction site
  • Rapidly and precisely generate correctly oriented constructs and inserts
  • Work with PCR that employ any thermostable polymerases


    • Applications
    The GenScript CloneEZ® PCR Cloning Kit can be used in a variety of applications:
  • PCR cloning
  • HTP PCR cloning
  • Joining of DNA fragments

    • Order:

     Cat.No.NameSizePriceQuantity
     L00339 CloneEZ® PCR Cloning Kit  24 rxns$ 175.00

    Technical Manual


    Components:

    Components L00339
    CloneEZ® Enzyme (5 U/μl) 50 μl 
    10X CloneEZ® Reaction Buffer 100 μl 
    pUC57 Linearized (KpnI/HindIII,100 ng/μl) 10 μl 
    1-kb Control Insert (100 ng/μl) 10 μl 

    MSDS:  20081029013928  (PDF)

    The inserts were detected by PCR screening using single colonies. Some of the results are shown in the figure below.


    1
    		 Custom Recombinant Protein Services : Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    		 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    		 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    		 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    		 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.