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CloneEZ® Kit


Kit Description:

The GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. — Using our proprietary CloneEZ® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C). br>
Key Features:

The CloneEZ® PCR Cloning Kit offers flexible and quick directional cloning and enables users to::
  • Clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases
  • Provide up to 12 kb PCR cloning at any restriction site
  • Rapidly and precisely generate correctly oriented constructs and inserts
  • Work with PCR that employ any thermostable polymerases


    • Applications:

    The GenScript CloneEZ® PCR Cloning Kit can be used in a variety of applications:
  • PCR cloning
  • HTP PCR cloning
  • Joining of DNA fragments
    • Order:

     Cat.No.NameSizePriceQuantity
     L00339 CloneEZ® Kit  24 rxns$ 175.00

    Technical Manual:

    Components:

    Components L00339
    CloneEZ® Enzyme (5 U/μl) 50 μl 
    10X CloneEZ® Reaction Buffer 100 μl 
    pUC57 Linearized (KpnI/HindIII,100 ng/μl) 10 μl 
    1-kb Control Insert (100 ng/μl) 10 μl 

    MSDS:  20081029013928  (PDF)

    The inserts were detected by PCR screening using single colonies. Some of the results are shown in the figure below.


    1
    		 Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
    2
    		 PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
    3
    		 Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
    4
    		 Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.