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High-Stability PCR Kit

Description

GenScript High-Stability PCR Kit contains Taq DNA polymerase, 10X buffer, and 10 mM dNTP, all specially formulated for increased stability using our proprietary technology. The kit can be stored at 4°C for over six months or at room temperature (25°C) for one month without significant loss of activity. The stabilizers in the buffers also increase the stability of Taq DNA polymerase at higher temperature (72°C), increasing the DNA yield of PCR amplifications of long DNA. This kit facilitates quick and easy PCR setup without any need for thawing. This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time.

Key Features

• High Stability: The kit remains stable for more than six months when stored at 4°C or for one month when stored at room temperature (25°C) without significant activity loss.
• Terminal Transferase Activity: Taq DNA polymerase has terminal transferase activity that results in the addition of a single nucleotide (adenosine) at 3´-end of the extension product.
• Increased PCR Yield: The green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR yield when amplifying long DNA.

Order:

Cat.No. Name Size Price Quantity   L00342 High-Stability PCR Kit  1 kit $ 64.00

Technical Manual Download PDF fileTM0285 Components: Components L00342 Green Taq DNA polymerase  100 μl  10X Reaction Buffer  1.5 ml  Stabilized dNTP  300 μl  Protocol  1  COA:  L00342_R20051001  (PDF) COA:  L00342_R60541007  (PDF) COA:  L00342_R60101104  (PDF) Examples: The green Taq DNA polymerase increases PCR yield when amplifying long DNA. Figure 1. A 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (in this case, the elongation time was eight minutes), the green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR DNA yield significantly. The green Taq DNA polymerase can be stored at 37°C for 14 days without loss of activity. Figure 2. A 2-kb 23S rRNA fragment from E. coli genomic DNA was amplified using green Taq DNA polymerase stored at -20°C and 37°C, respectively. The green Taq DNA polymerase can be stored at 37°C for 14 days without significant activity loss.

Related Products:   Code Name Size Price Quantity E00043  Enzyme : Green Taq DNA Polymerase  1000 U  $ 60.00 C01689  dNTP : Stabilized dNTP Mix, 10 mM each  0.5 ml  $ 29.00 B0005  10 x Taq Buffer  1.5 ml  $ 10.00

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