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Ultra Protein A Resin

Description

GenScript Ultra Protein A Resin is an affinity chromatography medium designed for easy, one-step and large-scale purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Compared to Protein A Resin (GenScript, L00210), the recombinant protein A ligand is more densely coupled to 4% highly cross-linked agarose, resulting in much higher static binding capacity – 30 to 40 mg hIgG/ml settled resin. The dynamic binding capacity of Ultra Protein A Resin will vary depending on several factors such as target antibody, flow rate etc.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Total Volume:

5 ml settled resin (10 ml 50% slurry)

Key Features

Strong Versatility: This product has a broad IgG binding spectrum.
High Binding Capacity: The capacity of Ultra Protein A Resin is 30-40 mg hIgG per ml of resin
Extra Convenience: The product can be used with cross-linked agarose media.

Order:

EXAMPLE:

Protein A Resin- Higher Binding Capacity

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PROTOCOL: 20100420081350 (PDF)
COA: L00400_R60431103 (PDF)


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