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pRNA-CMV3.1/Puro

Cat. No.	Size	Price
SD1233-10 ug	10 ug	$ 225.00
Full Name	pRNA-CMV3.1/Puro

Human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on RNA polymerase II system, CMV promoter drives higher-level constitutive expression of genes in a variety of mammalian cell lines, compared with RNA polymerase III based promoters such as U6 and H1. In this vector, CMV promoter drives the expression of siRNA, and SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Hind III sites. siRNA sequence can be confirmed by DNA sequencing using Reverse Sequencing Primer DA0012 (5’-TAGAAGGCACAGTCGAGG-3’)

Storage Buffer and Concentration: Store at -20°C

Download Protocol: tm0172

Forward Sequencing Primer:
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer:
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map:
Puromycin: 1598-2197
Polylinker: 584-651
CMV Promoter: 1-583
SV40 Promoter: 1267-1598
pUC ori: 2896-3536
Ampicillin: 3684-4544

Vector Sequence and Restriction Enzyme Map
PRODUCTINFO:  20051023234759  (PDF)
PROTOCOL:  20050718094354  (PDF)

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