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pRNAT-H1.4/Retro

Cat. No.	Size	Price
SD1253-10 ug	10 ug	$ 350.00
Full Name	pRNAT-H1.4/Retro

Description: pRNA-H1.1/Retro siRNA expression vector is compatible with Clontech Retro-X Expression System. An H1 promoter drives the siRNA expression with the siRNA insert cloned between MluⅠ and XhoⅠsites. The vector contains a hygromycin resistance gene under the control of cytomegalovirus (CMV) promoter* for establishing stable cell line. The vector uses CMV enhancer/promoter joined MSV 5’LTR (CMV/MSV 5'LTR) and MSV 3'LTR for viral transcription and packaging.

Storage Buffer and Concentration: Store at -20°C

Download Protocol: TM0187

Forward Sequencing Primer:
DA0025: SD1241 Forward (TGTAGGTTTGGCAAGCTAGC)

Reverse Sequencing Primer:
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Detailed Map:
5' LTR: 1-727
3' LTR: 5460-5755
psi+: 757-1566
ColE1 ori: 6456-7342
SV40 ori & promoter: 6012-6356
IRES: 2975-3555
Polylinker: 5288-5311
H1 Promoter: 5312-5411
CMV Promoter: 1604-2132
cGFP: 2247-2966
Hygromycin: 3593-4613
Ampicillin: 7402-8262

Vector Sequence and Restriction Enzyme Map

Publications used this GenScript product:

• Dympna Harmey, Anthony Smith, Scott Simanski, Carole Zaki Moussa, and Nagi G. Ayad. The anaphase promoting complex induces substrate degradation during neuronal differentiation. J. Biol. Chem. Feb 2009; 284(7): 4317 - 4323